免疫抑制比浊法及离子交换层析微柱对糖化.doc

免疫比浊法与离子交换层析微柱法测定糖化血红蛋白的方法学比较 王娟 张晓兵 杨新 （第三军医大学附属西南医院检验科 重庆400038） 摘要：目的 ：免疫比浊法与离子交换层析微柱法定量检测HbA1C方法学比较。方法：采用免疫比浊法和离子交换层析微柱分别对同一标本做HbA1C含量检测，比较两种方法的精密度、准确性、线形范围、相关性和单位样本测定时间。结果：免疫比浊法精确度和重复性较好,与离子交换层析微柱法没有显著性差异（测得正常人和糖尿病患者抗凝血批内CV值免疫比浊法为1.40％和1.15％，离子交换层析微柱法为1.5％和1.3％；日间CV值免疫比浊法为2.82％和2.57％，离子交换层析微柱法为2.97％和2.74％）；用免疫比浊法和离子交换层析微柱法测得正常组HbA1C 50 例分别为5.32±0.41％和4.87±0.37％ ，而糖尿病A组33例HbA1C分别为9.41±0.42％、9.23±0.41％ 和糖尿病B组HbA1C17例分别为11.15±0.47％、11.02±0.43％，并且正常人与糖尿病相比有着显著差异（P＜0.01）。免疫比浊法与离子交换层析微柱法具有较高相关性（r＝0.9540 ，P＜）HbA1C均具有较高的精确度和重复性，但免疫比浊法操作更简单、要求标本量少，测量时间短，可作为自动化检测HbA1C的首选方法。 关键词：糖化血红蛋白、免疫比浊法、离子交换层析微柱　　 Comparison between an immunoturbidimetric Assay and ion exchange chromatography for Hemoglobin A 1c WangJuan，Zhang Xiao-bing,YangXin(Department of Laboratory medicine , Southwest Hospital, Third Military Medical University，Chongqing 400038) Abstract: Objective: Comparison of glycosylated hemoglobin determination by immunoturbidimetry assay and ion exchange chromatography method. Methods: using immunoturbidimetry assay and ion exchange chromatography to measure the same groups of sample ，compare the result.Results：The accuracy and repetitive are better,and has no significant difference between ion exchange chromatography(p＞0.05). By immunoturbidimetry assay,the HbA1C levels in blood of 50 normal aldonors and 33 diabetics in group A and 17 diabetics in group B on admission were 5.32±0.41％ and 9.41±0.42％ and 11.15±0.47％, respectively.A significant difference was found between normal and diabetes mellitus groups(P0.01).There was a positive correlation between the results of immunoturbidimetry assay and ion exchange chromatography (r=0.9540, P0.01) Conclusion: The two methods have good accuracy and repetitive determinating glycosylated hemoglobin, immunoturbidimetry assay is simpler and the sample is fewer.The methods is principal to be analyzed on an automaticated chemistry analyzer. Key words:glycosylated; hemoglobin A;immunoturbidimetry; ion exchange chromatography 糖