中山大学细胞生物学英文题库.doc

中山大学细胞生物学英文题库 1. You think you might have discovered a new organelle using electron electron microscopy, but you are not sure if its real or an artifact of your technique. What might you do to prove it is real? a. Look for the structure in many similarly prepared specimens from the same source. b. Look for the structure in many similar prepared specimens from different sources. c. Look for the same structure in specimens prepared using different staining protocols. d. Look for the same structure in specimens prepared using different (or no) fixatives. 2. Traditional genetic approaches to problems of cell biology have followed the path of information from gene to protein. Biochemical approaches have traced proteins back lto the DNA that encodes them, i.e., from protein to gene. This chapter has given you the basics to follow either path. Assume you are studying a problem relating to cell function. Briefly outline the basic steps you would take if yo u were starting with a gene and trying to deduce the function of the protein. Now outline the steps that you would take to get from a specific protein to a sequenced, isolated gene. 1. Indicate whether the following statement is true or false. If it is false, expolain why in Nuclease-chypersensitive sites in chromation are located in the linker DNA between nucleosomes.(F) 2. Short answer a. Evolutionary conservation of histone proteins. b. nuclear matrix 3. Suppose you discover a temperature-sensitive nutant whose nucleus failed to accumulate certain nuclear protein atan elevated (restrictive) temperature, but continued to accumulate other species. What conclusions might you draw about nuclear localization and the nature of this mutation? Cells possess more than one NLS. receptor that recongnize different localization sequences, and that the mutation was occuring in a gene that encoded one of these NLS receptor species. 1. Fill in the blanks. A functional chromosome regrires the DNA sequence element at least one _______ t