粉葛的组织培养和成苗和快繁研究.doc

粉葛的组织培养和成苗技术研究 摘要：MS + NAA 0.1mg /L + 6-BA 0.3mg /L为培养基，获得的无菌苗生长健壮，枝叶繁茂，是成苗培养的最适培养基。2）最佳不定根诱导培养基为MS + NAA 0.3mg/L + IBA 0.3 mg /L，生根率达100%，低浓度的NAA能有效促进不定根数量的增多，主根增粗，增长，增多等作用。结论 为粉葛的快速繁殖和脱毒研究和中草药产业技术提供重要的后期研究基础。关键词粉葛; 组织培养; 成苗; 生根 Study on the seedling technology and tissue culture of Radix Puerariae Abstract: Objective urvival and rooting by use the material of the terminal bud and stem segments with buds, establishing The aseptic Department, exploring the Pueraria thomsonii tissue culture seedling Result show that:1)take MS + NAA 0.1mg /L + 6-BA 0.3mg /L as medium, to get No-vaccine robust growth, lush foliage,which is optimal medium for seedling cultivation. 2)The Best adventitious root induction medium for MS + NAA 0.3mg/L + IBA 0.3 mg /L, Rooting rate of 100%, The low concentration of NAA is effective in promoting adventitious root number increased, the role of Taproot thickening, growth, and increasing. Conclusion This study would provide important post-research base for the rapid propagation of Pueraria thomsonii and virus free studies and Chinese herbal medicine industrial technology. Keywords: Pueraria thomsonii; Tissue culture；Into seedlings；Rooting  目录 1…………………………………………………………………………………1 1.1粉葛的概况………………………………………………………………………1 1.2粉葛成苗的研究意义……………………………………………………2 2材料与方法…………………………………………………………………………2 2.1实验材料…………………………………………………………………………2 2.2培养方法…………………………………………………………………………2 2.3外植体的消毒……………………………………………………………………2 2.4无菌苗的获得……………………………………………………………………2 2.5成苗培养………………………………………………………………………… 2.6生根培养…………………………………………………………………………3 3结果与分析…………………………………………………………………………4 3.1不同植物生长调节剂对成苗的影响……………………………………………4 3.2不同植物生长调节剂对粉葛试管苗生根的影响………………………………6 4讨论…………………………………………………………………………8 4.1植物生长调节剂对成苗培养的影响……………………………………………8 4.2植物生长调节剂对诱导生根的作用……………………………………………8………………………………………………………………………………9 参考文献………………………………………………………………………………………………………………………………………………………………11 粉葛的组织培养和成苗技术研究 1前言 1.1粉葛的概况 粉葛是豆科（Leguminosae）葛属（PuerariaDC.）植物甘葛藤（Pueraria thomsonii Benth.）的干燥根[1]。葛始载于《神农本草经》,列为中品,历代医药著作均有记载,而《神农本草目》[2