明胶_丝素_壳聚糖微载体培养肝细胞的比较研究.pdf

2010 50 12 # # ) ,, ,,, * (南方医科大学珠江医院, 广州510280) : ) , , Cu ltisp er S ) 50 m l, ) Cu ltisp er S CL-1, , CL-1 Cultisp er S , ) 9, ) 5. 7 @ 106 /m l, Cultisp er S(P 0. 01) 10, ) Cultisp er S(P 0. 01) Cultisp er S, ) CL-1 : ; ; ; ; : R318. 14 : A : 1002-266X( 2010) 12-0003-04 M icrograv ity culture of hepatocyte on silk-fibroin-chitosan/gelatin macroporous m icrocarrier ZHANG Zh,i GONG Du-hu,i ZHOUH uan-cheng, L I Zhi-guo, PAN M ing-x in, GAO Yi (Zhuj iang H osp ital, outhernM ed ical University, Guangzhou 510280, P. R. China) Ab stract: Object ive To compare t e effect of silk-fibroin-c itosan ( SFC) m acroporousm icrocarrier on epatocyte cu-l ture underm icrogravity conditionsw it Cultisp erS. M ethods SFC compositem icrocarrierswere prepared bym et od ofwa- ter- in-oil emulsion w it add itional freezing and lyop ilization. CL-1 cellswere cu ltured on SFC and Cultisp er S respectively in a rotational cell culture system ( RCCS) at a volum e of 50 m .l Dynam icmorp ological observation, cell counting, album in secretion and urea synt es is analysiswere app lied t roug out cu lture. Results CL-1 cells cu ltured in am anner ofm onolayer on Cultisp er Sw ereas cells on SFC w ere multilayer. C ell accountings reac ed peak at t e 9 t day and t e m axmi um cell 6 density was 5. 7 @ 10 /m l on SFC, w ic was significantly ig er t an t ose on Cu ltisp er S (P 0. 01). A lbum in and urea in supernatant of SFC w as also ig er t an t at of Cultisp er S since 10t day (P 0. 01). Con clu sions SFC m acroporous