虫草素的微波-超声波协同提取-食品科学.pdf

86 2010, Vol. 31, No. 10 食品科学 ※工艺技术 王 陶，李 文，陈宏伟，陈小龙，刁进进 (徐州工程学院食品(生物)工程学院，江苏徐州 221008) ：研究蛹虫草菌丝体中虫草素的提取工艺及检测方法。确定微波- 超声波协同提取法为虫草素提取的最佳方 法，依据单因素和正交试验获得最佳的提取工艺：乙醇体积分数70% 、提取功率200W 、提取时间110s、料液 比1:240(g/mL)。此时虫草素含量达9.228mg/g 。采用紫外分光光度法测定样品中虫草素含量，回收率为97.41%～ 99.60% ，RSD 为1.469%，准确度和精密度都较高，是一种方便、成本较低的检测方法。 ：蛹虫草；虫草素；微波- 超声波协同提取；紫外分光光度法 Microwave-Ultrasonic Assisted Extraction of Cordycepin from Cultured Mycelia of Cordyceps militaris WANG Tao ，LI Wen ，CHEN Hong-wei ，CHEN Xiao-long ，DIAO Jin-jin (College of Food (Biology) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China) Abstract ：Single factor and orthogonal array design methods were used to investigate an optimal procedure for the extraction of cordycepin from cultured mycelia of Cordyceps militaris with the assistance of microwave combined with ultrasonic. Besides, a UV spectroscopic method was developed for the assay of cordycepin and its figures of merit were evaluated. Results showed that the optimal values of crucial technological parameters affecting cordycepin yield were as follows: ethanol concentration 70%, microwave power 200 W, material/liquid ratio 1:240 (g/mL) and extraction time 110 s. Under such conditions, a cordycepin yield of up to 9.228 mg/g was obtained. The developed analytical method exhibited mean recoveries ranging from 97.41% to 99.60% of 3 replicates for cordycepin in a real sample spiked at 3 levels and both high accuracy and precision so as to provide a convenient and cost saving method for the assay of cordycepin. Key words ：Cordyceps militaris；cordycepin ；microwave-ultrasonic assisted extraction ；UV spectrophotometry 中图分类号：Q939.96 文献标识码：A 文章编号：1002-6630(2010)10-0086-05 蛹虫草又名北冬虫夏草、蛹草、蛹草菌、北虫草