a functional high-content mirna screen identifies mir-30 family to boost recombinant protein production in cho cells文档.pdf

Biotechnology Biotechnol. J. 2014, 9, 1279–1292 DOI 10.1002/biot.201400306 Journal Research Article A functional high-content miRNA screen identifies miR-30 family to boost recombinant protein production in CHO cells 1,2 1 1,3 1 1 1 Simon Fischer , Theresa Buck , Andreas Wagner , Carolin Ehrhart , Julia Giancaterino , Samuel Mang , Matthias Schad1 1 4 1 1 , Sven Mathias , Armaz Aschrafi , René Handrick and Kerstin Otte 1 Institute of Applied Biotechnology, University of Applied Sciences Biberach, Biberach, Germany 2 Faculty of Medicine, University of Ulm, Ulm, Germany 3 Faculty of Natural Sciences, University of Ulm, Ulm, Germany 4 Department of Neuroinformatics, Donders Institute for Brain Function, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands The steady improvement of mammalian cell factories for the production of biopharmaceuticals is Received 06 MAY 2014 a key challenge for the biotechnology community. Recently, small regulatory microRNAs (miR- Revised 12 JUN 2014 NAs) were identified as novel targets for optimizing Chinese hamster ovary (CHO) production Accepted 23 JUL 2014 cells as they do not add any translational burden to the cell while being capable of regulating entire Accepted physiological pathways. The aim of the present study was to elucidate miRNA function in a recom- article online 24 JUL 2014 binant CHO-SEAP cell line by means of a genome-wide high-content miRNA screen. This screen revealed that out