development of a sensitive molecular detection assay for mango malformation disease caused by fusarium mangiferae.发展一个敏感的分子检测化验芒果畸形病镰刀菌素引起的mangi推荐.pdf

Biotechnol Lett (2016) 38:835–840 DOI 10.1007/s10529-016-2048-9 ORIGINAL RESEARCH PAPER Development of a sensitive molecular detection assay for mango malformation disease caused by Fusarium mangiferae Jingbo Wu . Feng Liu . Rulin Zhan . Guoping Li . Yanlong Zhao . Chang Jinmei . Yanbiao He Received: 4 November 2015 / Accepted: 18 January 2016 / Published online: 23 February 2016 Springer Science+Business Media Dordrecht 2016 Abstract F. mangiferae and other species of Fusarium both Objectives To develop a sensitive and specific in vitro and in vivo. It can detect as little as 10 pg molecular assay for detection of mango malformation fungal DNA from the DNA of mango’s tissues. disease (MMD), which is caused primarily by Fusar- Conclusions Our assay provides a practical method ium mangiferae. for the early diagnosis so that proper prevention of the Results We screened 100 ISSR primers and identi- mango malformation disease can be developed. fied one (UBC888) that directed the stable amplifica- tion of a specific gene fragment of 479 bp (GenBank Keywords Abnormal plant growth Fruit tree accession number KJ526382). Based on the DNA diseases Hyphomycetes Mangifera indica Mango sequence of this fragment, a pair of SCAR primers Mango disease Molecular markers (W342 and W1772) were designed to amplify another gene fragment of 1376 bp (GenBank accession num- ber KJ526383), demonstrating the successful conver- sion of an ISSR marker to a SCAR marker. An Introduction effective and simple detection assay for MMD was established based on this pair of PCR primers, with a Mango malformation disease (MMD) is a common high level of specificity