enhanced transient recombinant protein production in cho cells through the co-transfection of the product gene with bcl-xl推荐.pdf

BiotechnologyBiotechnology Biotechnol. J. 2014, 9, 1164–1174 DOI 10.1002/biot.201300468 JournalJournal Research Article Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL 1,2 2 2 2 1 Matthew P. Zustiak , Lisa Jose , Yueqing Xie , Jianwei Zhu and Micheal J. Betenbaugh 1 Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA 2 Biopharmaceutical Development Program, SAIC-Frederick, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA Transient gene expression is gaining popularity as a method to rapidly produce recombinant pro- Received 31 OCT 2013 teins in mammalian cells. Although significant improvements have been made, in terms of expres- Revised 11 FEB 2014 sion, more improvements are needed to compete with the yields achievable in stable gene expres- Accepted 05 MAR 2014 sion. Much progress has come from optimization of transfection media and parameters, as well Accepted as altering culturing conditions to enhance productivity. Recent studies have included cell lines article online 07 MAR 2014 engineered for apoptosis resistance through the constitutive expression of an anti-apoptotic pro- tein, Bcl-xL. In this study, we examine an alternative method of using the benefits of anti-apoptot- ic gene expression to enhance the transient expression of biotherapeutics, namely, through the co- transfection of Bcl-xL and the product-coding gene. CHO-S