METHODS IN ANIMAL CELL CULTURE - Home - Dieter H#252;lser：方法在动物细胞培养-家居-迪特尔H和# 252；激光.pdf

1 METHODS IN ANIMAL CELL CULTURE Dieter F. Hülser Institute of Biology, Department of Biophysics, University of Stuttgart D 70 0 Stuttgart, Germany 1 Principles 1.1 Development of cultured cells 1.2 Selection of cells 1.3 Cell cycle and growth curves 1.4 Cell profiling 1. Ethical rules 2 Techniques 2.1 Preparation of primary and continuous cultures 2.1.1 Monolayer cultures 2.1.2 Suspension cultures 2.1.3 Three-dimensionally growing cultures 2.2 Cell culture media 2.3 Equipment 2.3.1 Vessels 2.3.2 Appliances 2.4 Safety, Biohazards, 2. Storage 3 Applications 3.1 Cellular regulations 3.2 Cancer research 3.3 Production 4 Perspectives 2 Key Words Cell cloning Generation of a colony from a single cell. Subculturing results in a cell strain. Cell cycle Ordered sequences (G , S, G , and M) of cellular syntheses between two cell 1 2 divisions. Cell line Subcultured primary cultures. A cell line can be finite or continuous. Cell strain Cell line which has been purified by physical separation, selection, or cell cloning. Continuous cell line Indefinite proliferation. This immortalization of a cell line may be induced by a viral gene transfer or was already acquired by some cancer cells before cultivation. Primary culture Freshly isolated cells in culture until the first passage into a subculture. Serum Blood fluid without cells and clotting factors. Suspension Culture Cells proliferate isolated from each other when suspended in growth medium. Tissue Culture Accustomed term for the cultivation of animal cells. Originally: Fragments of tissues maintained in vitro. 3 Animal cell cul