non-invasive in vivo imaging of cardiac stem_progenitor cell biodistribution and retention after intracoronary and intra.心脏stem_progenitor细胞体内非侵入性成像bio文档.pdf

Collantes et al. J Transl Med Journal of DOI 10.1186/s12967-017-1157-0 Translational Medicine RESEARCH Open Access Non-invasive in vivo imaging of cardiac stem/progenitor cell biodistribution and retention after intracoronary and intramyocardial delivery in a swine model of chronic ischemia reperfusion injury 1† 2† 1 3 2 María Collantes , Beatriz Pelacho , María José García‑Velloso , Juán José Gavira , Gloria Abizanda , 4 4 4 1 5 2 Itziar Palacios , Luis Rodriguez‑Borlado , Virginia Álvarez , Elena Prieto , Margarita Ecay , Eduardo Larequi , 1* 6* Iván Peñuelas and Felipe Prósper Abstract Background: The safety and eicacy of cardiac stem/progenitor cells (CSC) have been demonstrated in previous preclinical and clinical assays for heart failure. However, their optimal delivery route to the ischemic heart has not yet been assessed. This study was designed to determine by a non‑invasive imaging technique (PET/CT) the biodistri‑ bution and acute retention of allogeneic pig CSC implanted by two diferent delivery routes, intracoronary (IC) and intramyocardial (IM), in a swine preclinical model of chronic ischemia–reperfusion. Methods: Ischemia–reperfusion was induced in six Goettingen hybrid minipigs by 90 min coronary artery occlu‑ sion followed by reperfusion. Thirty days later, animals were allocated to rece