fast photovoltage measurements in photosynthesis. ii. experimental methods推荐.pdf

Fast Photovoltage Measurements in Photosynthesis. 11. Experimental Methods HANS-WILHELM TRISSL* and KARSTEN WULF Abt. Biophysik, Fachbereich Biologie/Chemie, Universitat Osnabruck, Barbarastrane 11, D-49069 Osnabruck, Germany SYNOPSIS Fast photovoltage measurements in the pico- and nanosecond time range represent a valuable tool for the study of excitation energy trapping, kinetics of charge separation, and the location of intermediary acceptors in the photosynthetic reaction center. How- ever, data recording and data analysis are complex. In a preceding article (Wulf and Trissl, Biospectroscopy,1 (1995),pp. xx-xx) we have described the theoretical fundamen- tals of data analysis. Here we describe the experimental set-up, calibration procedures, and strategies for the determination of the parameters involved. Limitations of the method will be discussed. 01995John Wiley Sons, Inc. INTRODUCTlON cal materials in the past decades, reviewed in Refs. 4-6. The different experimental methods are inti- The primary photosynthetic reactions as there are mately related to the way the orientation is the transfer of excitation energy from antenna pig- achieved. Open membrane sheets can be oriented ments (A:) to the primary donor, P, of the reaction either by adsorption to surfaces7, (mostly thin center (RC) and the transfer of an electron from films) or by application of electric field^.^,^ Vesicles P* to primary and secondary acceptors are conve- containing chromoproteins may be fused with niently detected