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fast photovoltage measurements in photosynthesis. ii. experimental methods推荐
Fast Photovoltage Measurements in Photosynthesis.
11. Experimental Methods
HANS-WILHELM TRISSL* and KARSTEN WULF
Abt. Biophysik, Fachbereich Biologie/Chemie, Universitat Osnabruck, Barbarastrane 11, D-49069 Osnabruck, Germany
SYNOPSIS
Fast photovoltage measurements in the pico- and nanosecond time range represent a
valuable tool for the study of excitation energy trapping, kinetics of charge separation,
and the location of intermediary acceptors in the photosynthetic reaction center. How-
ever, data recording and data analysis are complex. In a preceding article (Wulf and
Trissl, Biospectroscopy,1 (1995),pp. xx-xx) we have described the theoretical fundamen-
tals of data analysis. Here we describe the experimental set-up, calibration procedures,
and strategies for the determination of the parameters involved. Limitations of the
method will be discussed. 01995John Wiley Sons, Inc.
INTRODUCTlON cal materials in the past decades, reviewed in Refs.
4-6. The different experimental methods are inti-
The primary photosynthetic reactions as there are mately related to the way the orientation is
the transfer of excitation energy from antenna pig- achieved. Open membrane sheets can be oriented
ments (A:) to the primary donor, P, of the reaction either by adsorption to surfaces7, (mostly thin
center (RC) and the transfer of an electron from films) or by application of electric field^.^,^ Vesicles
P* to primary and secondary acceptors are conve- containing chromoproteins may be fused with
niently detected
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