生物科学毕业论文设计-银杏内生真菌分离培养及生物活性测定[精品].doc

生物科学毕业论文设计-银杏内生真菌分离培养及生物活性测定 化学工程与食品科学学院毕 业 论 文设 计题 目 银杏内生真菌的分离培养与生物活性测定 专 业 生物科学 班 级 0911班 学生姓名 岳继萍 学 号 2009124121 指导老师 阎 华 二〇一三 年 五 月摘 要内生真菌是一类生长于活体植物组织中的特殊的真菌研究相对较少生长于独特环境中具有药用价值树龄长或有地域特色的植物中一般都能分离出新的内生菌而新种或罕见菌种又能为寻找新型生物活性物质提供前景银杏是我国特色珍稀植物具有活化石之美称银杏在生长期间很少感染病虫害寿命极长因此具有很好的挖掘新型内生真菌的潜力本研究采集湖北省襄阳市湖北文理学院内银杏枝条和树叶采用组织分离法分离其中的内生真菌筛选具有杭菌性和抗氧化活性的菌株并通过形态特征进行初步鉴定对其中三株株杭菌活性显著且稳定的不产孢菌进行形态学研究和分子解析对培养条件和培养基组成进行优化进而分离其发酵产物中的抗菌活性物质并进行结构鉴定为进一步工业化生产应用提供工作基础和依据银杏 又名白果 外种皮 及果仁的提取物对水稻纹枯病菌黄瓜炭疽病菌和番茄青枯病菌等有明显的抑制生长作用[ 6 8] 本研究以银杏为材料 AbstractEndophytic fungi is a kind of special fungus growth in living plant tissuewhitch research is relatively smallit grew up in special environment with medicinal value and old long or have regional characteristics of plants in general can be new endogenous bacteria was isolated while new species and rare species can provide prospects for search for new bioactive substances Ginkgo is characteristics of rare plants in our country has Living fossils good name Ginkgo rarely infection diseases and pests during growth its life is very long so it is good to dig up the potential new type of endophytic fungi this study collected hubei province of hubei liberal arts college in ginkgo biloba leaves and branches of xiangyang city using chromatography separation of endophytic fungi bacteria screening with antimicrobial properties and antioxidant activity of strains and preliminary identification of morphological characteristics Significant activity of three bacteria antimicrobial properties and stable does not produce robe studied the morphological and molecular analysis optimize the culture conditions and medium composition antibacterial activity of the fermentation substances into miseparation and structural identification for further industrialized production application work provide dhi and basis Ginkgo also known as ginkgo is our country unique rare and precious tree species and the