05-病毒学virus巴斯德研究所.ppt

Re-assortant Segmented virus Phenotype mixing Psued virus technology Complementation x x x x x x x x x x abort rescue ts mutant Temperature sensitive mutant = point mutation x x x x x 39oC 37oC x abort Functional protein Non-functional protein * Replication of DNA Viruses IPS Tetsuya Toyoda Feb. 28, 2006 1. Replication 2. Transcription DNA RNA Protein DNA polymerases DNA depedent RNA polymerases Central Dogma DNA RNA Protein Prion RNA viruses Viroids SiRNA RNA dependent RNA polymerase PrPsc Reverse transcriptase Retroviruses HBV Ribozyme Ribozyme RNA World: Unique Gene Expression lymphocyte brain heart lung kidney spleen intestine Infected monocyte Primary viremia Secondary viremia Subcutaneal haemangitis Skin rush Free virions Local lymphnode antibodies Systemic lymphnodes SSPE virus Droplet infection Koplik spot Local infection Systemic infection lypmocytepenia Replication of virus in vivo Measles virus mRNA NS1 PB1-F2 adsorption Parent virus endocytosis Membrane fusion endosome uncoating nucleus HA、NA、M2 Transcription, replication translation M1、NS2 budding release New virus Replication of virus in the infected cell Influenza virus Stream of genetic information of DNA viruses ＜Group 1 viruses＞ ＜Group 2 viruses＞ ＜Group 3 viruses＞ ＜Group 4 viruses＞ ＜Group 5 viruses＞ ＜Group 7 viruses＞ dsDNA(+) mRNA dsDNA(+) ssDNA(+) dsRNA(+) ssRNA(+) ssRNA(-) ssRNA(-) ssDNA(-) ssRNA(+) dsDNA(+) mRNA Stream of genetic information of RNA viruses ＜Group 6 viruses＞ ssRNA(+) ssRNA mRNA mRNA （±） By Baltimore Transcription, Replication in general enzyme cis-factor trans-factor (cis-element) Transcription: mRNA synthesis transcriptase promoter transcription factor terminator enhancer, silencer