翼首草药材指纹图谱研究.doc

藏药翼首草的HPLC指纹图谱研究 张小梅1,2，柯秀梅，2，励娜2，王云红2，梁旭明2* （1.成都中医药大学,成都 611137； 2.重庆市中药研究院 重庆市中药资源重点实验室,重庆 400065） [摘要] 目的:建立藏药翼首草的高效液相色谱指纹图谱，并对不同产地翼首草的指纹特征进行比较，为其质量评价提供新的方法。方法:采用HPLC 测定了10 批不同产地的翼首草样品。Boston Symmetrix色谱柱（4.6 mm×2505 μm）甲醇-0.1mo·L-1乙酸铵溶液（819）流速0.8 m·min-1，检测波长210 nm，柱温302004A版）》建立了翼首草药材的指纹图谱共有模式，标出了11个特征峰。10批样品的相似度均较高。结论:该方法具有较好的重复性、精密度、稳定性，可为翼首草的质量控制提供科学依据。 关键词：藏药翼首草；高效液相色谱；指纹图谱 中图分类号：R283.1 文献标识码：A 文章编号： HPLC Fingerprint of Tibetan Medicine Pterocephali Herba ZhANG Xiao-mei1,2, KE Xiu-mei 1,2, LI Na2, WANG Yun-hong2, LIANG Xu-ming2* (1.Chengdu University of Traditional Chinese Medicine（TCM）, Chengdu 611137, China; 2. Chongqing Academy of Chinese Traditional Materia Medica, Key Laboratory of Chongqing TCM Resources ,Chongqing 400065, China) [Abstract] Objective: To establish HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare it of Pterocephali Herba from different place, provided a new methods for its quality evaluation. Method: Adoption the method of HPLC to determine 10 groups of Pterocephali Herba from different place. Chromatographic Column was Boston Symmetrix（250×4.6mm5μm）, mol·L-1 ammonium acetate solution(81:19),velocity was 0.8mL·min-1, the detection wavelength was 210nm, column temperature was 30℃. And compared the similarity of Pterocephali Herba from different place. Result: Taking advantage of USP fingerprint similarity evaluation system (2004A version) to establish common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba, and marked 101 characteristic peaks. The 10 groups samples had high similarity. Conclusion: This method had good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba. [Key words]: Tibetan Medicine Pterocephali Herba; HPLC; Fingerprint 翼首草进行质量评价11]，而这2种成分并非翼首草独有，药用植物中也同时含这2种成分年版HPLC技术建立了翼首草药材的指纹图谱并对其进行了系统研究，测定了10批不同产地的翼首草药材，建立了翼首草指纹图谱共有模式，为翼首草的质量控制及科学评价，提供试验依据。 2695型高效液相色谱仪Waters 2996检测器，Waters Empower化学工作站Waters公司），AEG-45SM型电