载氧脂质微泡的制备及特性研究-第三军医大学学报.DOC

201301061 论著 载氧脂质微泡的制备及特性研究 肖 欢1，唐 毅1，李 攀2，高 洋1，王 冬1，杨春江1（400014 重庆，重庆医科大学附属儿童医院超声科，儿科学重庆市重点实验室1；400010 重庆，重庆医科大学超声影像学研究所2） [摘要]目的 制备一种新型载氧脂质微泡，其理化性质、载氧能力、超声辐照对微泡释氧的影响以及兔肝显影效果。 方法 采用机械振荡法制备高效载氧的脂质微泡，观察60Co射线灭菌前后造影剂外观、形态、浓度、平均粒径和电位改变，检测超声辐照前后缺氧溶液溶解氧浓度（DO）的变化，并观察其对正常兔肝的显影效果。结果 自制载氧脂质微泡平均粒径约为（959.7±279.2）nm，粒径分布均匀，浓度约为（4.013±1.532）×109/m；60Co 射线灭菌前后，微泡形态，粒径大小、浓度及zeta电位无明显变化（P0.05）；5Hz、3.5W超声辐照，能够有效增加微泡释放氧气的能力；载氧脂质微泡能够明显增强兔肝实质回声。结论 自制载氧脂质微泡形态及性能稳定，能够有效载氧，超声辐照下微泡释氧能力明显增加，兔肝增强显影明显、持久，有望实现靶向运输氧气到达缺氧组织，提高组织或血氧浓度，为缺氧疾病提供一种新的治疗方式。 [关键词] 氧气；微泡；脂质体；载体；兔 Experimental Study on Preparation and Characteristics of Lipid-coated Oxygen Microbubbles Xiao Huan1，Tang Yi1，Li Pan2，Wang Dong1，Gao Yang1，Yang Chunjiang1（Department of Ultrasonography，Key Laboratory of Pediatrics in Chongqing，The Children’s Hospital of Chongqing Medical University，Chongqing 400014，China；Institute of Ultrasound Imaging，Chongqing Medical University，Chongqing 400010，China） [Abstract]Objective Prepare a kind of self-made lipid-coated oxygen microbubble， to study its characteristics ， the oxygen loading ability in vitro ， the affection of exchanging oxygen in the presence or in the absence of US ，and to assess its enhancement effects in contrast imaging on norma1 rabbit liver．Methods A kind of 1ipid microbubbles loading oxygen was prepared，and its appearance，concentration， mean diameter and zeta potential were detected before and after irradiance of 60Co Ray .The oxygen release kinetics were detected with or without ultrasound sonication .Five rabbits were examined to observe the enhancement time and intensity in the liver after microbbubles injected．Results The mean diameter was （959.7±279.2）nm and the concentration was（4.013±1.532）×109／m1， those indices were not changed significantly after sterilization with irradiance of 60Co Ray；The oxygen release kinetics could be enhanced after insonation with ultrasound at 3.5 W，5.0 KHz；Contrast imaging showed that the self-made lipid-coated oxygen microbubbles could