大野荞清除活性氧作用研究本科.doc

大野荞清除活性氧作用研究 摘 要：羟基自由基超氧阴离子自由基过氧化氢关键词： Scavenging of Reactive Oxygen Species by the Extraction of F. megaspartanium Abstract: This experiment used the method of nitro blue terra-zolium photochemical reduction, the system of fenton can produce the·OH, which can function with Saffron red T ,and then this system reduces the absorbance, H2O2 and ammonium molybdate can become the yellow soil thing and so on, to study the scavenging of the hydroxyl radical (·OH) , superoxide anion radicals (·O2 - ) and hydrogen peroxide ( H2O2 ) were estimated by aqueous extractions from various organs (flower, leaf, stem, root, seed)of F. megaspartanium. The results showed that: the cleaning effect of the flower on ·OH was more significant than the seed, the flower was not significant in difference between the leaf and stem; The root was not significant in difference in the scavenging effect of ·O2 - compared with the flower, but which was more significant than the leaf, seed and stem; The leaf was not significant in difference in the scavenging effect of H2O2 compared with the flower, but which was more significant than the root, seed and stem. Keywords: F. megaspartanium; ·OH; ·O2-; H2O2; Reactive oxygen species 目 录 引言 1 1. 2 1.1 供试材料 2 1.2仪器与试剂 2 1.3方法 2 2. 数据处理 5 3. 结果与分析 5 3.1大野荞各器官水提液对·OH的清除作用 5 3.2大野荞各器官水提液对·O2-的清除作用 6 3.3大野荞各器官水提液对H2O2的清除作用 6 4. 讨论 7 参考文献 致 谢 引言 荞麦属于蓼科(Polygonaceae)荞麦属(Fagopyrum)，全世界已见报道并正式命名的有23个种、3个变种、2个亚种和1个人工合成种，分为大粒和小粒两个组，以大粒组的荞麦较为重要[]。[2,3]。荞麦富含蛋白质、淀粉、粗纤维、维生素、矿物元素等，并含有一些其他粮食作物不含或少含的营养物质[4-7]。 活性氧是指生物体内有氧代谢过程中产生的或者在外界物理化学因素诱导下产生的含有氧原子并且氧化能力很强的数种物质的总称。主要有超氧阴离子自由基(·O2–)，羟基自由基(·0H)、过氧化氢(H202)等[]。正常情况下，活性氧的产生和清除是平衡的，但受到生物和非生物胁迫时自由基会产生过多或清除过慢，便会通过攻击生命大分子物质及各种细胞，造成机体的各种损伤，加速机体衰老进程并诱发各种疾病 [9]。需氧生物有一套抗氧化系统 ，主要包括抗氧化酶类 、修复酶类及一些生物分子。人体本身有一套清除自由基的系统SOD)、过氧化氢酶(CAT)、过氧化物酶(POD) 和抗坏血酸过氧化物酶(ASP)等对活性氧的清除较为重要。约90% 的·02一是由SOD 清除的，POD、 CAT、 ASP 则负责H2O2的清除[10]。但是，这会人的年龄增加而减弱，老年时达最低点的活性氧就会在体内与脂肪、蛋白质或者核酸等结合，形成氧化或过氧化物增加发生各种疾病的危险性。02一和·0H具有较强的清除作用，而对H2O2则无明显清除作用，并认为其研究结果对