磷脂脂肪酸谱图分析方法及其在微生物生态学领域的应用.PDF

23 8 　　　　　　 Vol. 23, No. 8 2003 8 ACTA ECO LOGICA SIN ICA Aug . , 2003 * 齐鸿雁 ,薛　凯,张洪勋 (,　 100085) : , 。, 、、。 : ; PLFA;;; Phospholipid fatty acid analysis and its applications in microbial ecology - , , - 　( Environmental B iotechnology Lab. , Research Center f or QI Hong Yan X UE Kai ZHAN G Hong Xun Eco-environmental Sciences , Chinese Academy of Sciences , B eij ing　 100085, Ch ina) .Act a Ecol og ica S inica , 2003, 23( 8): 15 6～ 1582. Abstract: The quantitative description of microbial comm unity is one of the difficult tasks for microbial ecologist. Conventional cultivation techniques cannot be used to fully characterize most soil microorganisms in respect that most of species cannot be cultured. Using phospholipid fatty acids ( PLFA) profiles to characterize microbial communities can overcome the limitation of traditional techniques, because it doesn t require the removal and the culture of the microbes. The membrane phospholipids exist in all living cellular m embrane. Th ey are not found in storage products or in dead cells by reason of rapidly degradation after cells dying . PLFA don t vary much in a cell , . which th ey form a part and keep reasonably constant amounts as cells occur in nature The analysis of the ester-linked fatty acids of th e phospholipids makes use of capillary gas chromatography and mass . , spect rometry With such technique th e component of each PLFA and the shift in PLFA pattern can be quantitatively analyzed. , PLFA profiles consisted of quantitative determination of each PLFA component and the shift in