胃癌差异表达蛋白的蛋白质组学分析-南华大学学报.PDF

14 Medical Science Journal of Central South China,January2015,Vol.43,No.1 DOI:10郾15972/ j郾cnki郾43鄄1509/ r郾2015郾01郾004 ·基础医学· 胃癌差异表达蛋白的蛋白质组学分析 1,2 1 1 1 1* 李素云 ,陈苏琼 ,谢远杰 ,张志伟 ,贺修胜 (1.南华大学肿瘤研究所,湖南衡阳421001;2.南华大学医学院解剖教研室) 摘摇 要:摇 目的摇 筛选与鉴定胃癌差异表达蛋白质分子。 摇 方法摇 采用定量蛋白组学方法,通过同位素 iTRAQ标记胃癌与正常胃黏膜组织总蛋白,然后用强阳离子交换/ 反相液相色谱(SCX/ nanoHPLC)进行电喷雾串联 质谱分析(ESI鄄MS/ MS),获得两组样本的多肽及相对丰度信息,通过数据库有哪些信誉好的足球投注网站与比对,鉴定差异表达蛋白质。 结果摇 共鉴定出319 个蛋白质,其中表达差异在2倍以上的88 个,在胃癌中呈高表达的12 个、呈低表达的76 个。 按蛋白质功能分为七大类:细胞骨架蛋白、分子伴侣、信号传导、生物代谢、凋亡,蛋白质合成与代谢类及其他蛋白 质。 摇 结论摇 成功筛选了胃癌差异表达蛋白质分子,这些蛋白质的表达改变,可能参与了胃癌的发生和发展。 关键词:摇 胃癌;摇 iTRAQTM 同位素标记相对和绝对定量;摇 多维液相色谱;摇 串联质谱 中图分类号:R735郾2摇 摇 摇 文献标识码:A ProteomicsAnalysisof Differential ExpressionProteinsinGastric Carcinoma LI Suyun,CHEN Suqiong,XIE Yuanjie,et al (Cancer Research Institute,University of South China,Hengyang,Hunan421001,China) Abstract:摇 Objective摇 To identify the differential expression proteins between gastric adenocarcinoma cells (GAC) and normal gastric adenoepithelial cells (NGAC),and to lookfor early diagnosis of Gastric carcinoma (GC )specific pro鄄 teins.摇 Methods摇 Thetotalproteinswereextractedfromthetissues.Thetotalproteinslabeledwithdifferent iTRAQstable isotopewere segregatedwith Nano鄄RPLC鄄MS/ MS,to obtain relative abundance of peptides information.The differential ex鄄 pression proteins were identified through SWISSPORT database.摇 Results摇 Through Quantitative proteomic analysis of GAC and NGAC,319 proteinswereidentifiedwith MS,88ofwhichweredifferential expressionproteins.Therewere 12 Up鄄 regulation and76 Down鄄regulation proteins in GAC.The identified proteins can be divided into seven groups according