胶质瘤动物模型推荐.ppt

转基因动物 通过实验手段把外源基因稳定地整合至基因组，并能遗传给后代的动物。 命名 C57BL/6J-TgN(CD8Ge)23Jwg 5、转基因动物(transgenic animals) * Thank you for your attention! * 胶质瘤动物模型 Glioma Animal model 1、实验诱发模型 1.1 化学诱发 1.2 病毒诱发 2、移植瘤模型 2.1 原位移植瘤 2.2 皮下移植瘤 3、转基因小鼠模型 Glioma Animal model * 首见于 1939 年 Seligman 把多环芳香烃类的甲基胆蒽制成丸剂植入小鼠脑内，诱发成胶质瘤和肉瘤。 从上世纪中叶起，因利用亲神经的烷化剂全身给药诱发神经系统肿瘤获得成功。 甲基亚硝基脲和乙基亚硝基脲有很高的中枢神经系统肿瘤诱发率，至今还在运用的 P494、C6、9L 和G422 等胶质瘤动物模型都是用上述类似方法制作成功。 1、实验诱发模型—化学诱发 * 病毒诱发有两类，一是核糖核酸病毒，如劳斯肉 瘤 病 毒； 二 是 脱 氧 核 糖 核 酸 病 毒 ， 如 腺 病 毒。 Bigner 等把浓缩的呼吸道合胞病毒（Respiratory Syncytial Virus，RSV）注射到新生犬脑内，经过一段潜伏期后全部发生脑胶质瘤。 1、实验诱发模型—病毒诱发 * 已建系的肿瘤细胞株 腹水瘤移植接种法 实体瘤移植接种法（1）小块接种法（2）肿瘤组织浆接种法 2、移植瘤模型—皮下移植瘤 移植瘤的接种方法 * 2、移植瘤模型—皮下移植瘤 （李承科，霍刚，郑履平，等.快速建立胶质瘤模型的研究，重庆医科大学学报[J].2008,33(1):73-76） * C6/SD rat C6/Wistar rat 9L/F344 rat CNS-1/ Lewis rat U87MG/Swiss-nu U87/BALB/nu-c 2、移植瘤模型—原位移植瘤 * 靶点坐标：大鼠右侧尾状核区，前囟中点前1.0mm，矢状缝右旁开3.0mm，硬脑膜下5mm * 2、移植瘤模型—原位移植瘤 Implantation technique Anesthesia was induced by inhalation of a mixture of oxygen with 5% halothane, followed by an intra-peritoneal injection of ketamine (87 mg/kg) and xylazine (13 mg/kg) for maintenance.Animals were then mounted on a stereotactic frame. Special care was used in the placement of the head in the frame to avoid sagittal angulation, so that the head was parrallel to the frame stand . A midline scalp incision was performed, followed by identification and exposure of the bregma. Using a 16-gauge needle, a burr hole was placed on the right frontal bone. A 25- microliter SGF syringe with a 27-gauge needle secured to the frame was used to infuse the cellular solution over a period of five minutes. The needle was then slowly withdrawn.Bone wax was applied to close the burr hole and the scalp was closed with a continuous one-layer resorbable suture. David Mathieu, Roger Lecomte, Ana Maria Tsanaclis, et al.Standardization anddetailed characteriz