亚细亚镰孢菌产碱性蛋白酶与致病力的相关性.PDFVIP

1150 江苏农业学报( Jiangsu J. of Agr. Sci. ), 2010, 26( 6): 1150~ 1155 张 旭, 马鸿翔, 温云平, 等. 亚细亚镰孢菌产碱性蛋白酶与致病力的 关性[ J]. 江苏农业学报, 2010, 26( 6): 张 旭, 马鸿翔, 温云平, 余桂红, 陆维忠 (江苏省农业科学院农业生物技术研究所, 农业部华东作物遗传改良与育种重点实验室, 江苏省农业生物学重点实验室, 江苏 南京 210014) : 选择了 6个不同致病力的亚细亚镰孢菌菌株, 提取纯化碱性蛋白酶, 利用蛋白酶活性电泳的方法对其 进行特性分析, 研究其致病力与其产碱性蛋白酶的 互关系。亚细亚镰孢菌产生的碱性蛋白酶最适反应温度为 30~ 40 , 最适反应 pH 值为 7. 5~ 85, 属于丝氨酸蛋白酶类型。菌株致病力强弱与其产碱性蛋白酶活性呈极显 著正 关。亚细亚镰孢菌产生的碱性蛋白酶可能与真菌的致病力有关。 : 小麦赤霉病; 亚细亚镰孢菌; 致病性; 碱性蛋白酶 + : S512. 1 10. 34 : A : 2010) Correlation between Pathogenicity and Alkaline Proteinase Activities of Fusarium A siaticum Isolates HANG Xu, MA H ongxiang, WEN Yunping, YU Guihong, LU W eizhong (Institute of B iotechonology, J iangsuA cademy of Agricultural Sciences, Key Laboratory of Crop enetic Imp rovement and Breeding in East China, M inistry of Agriculture, K ey Laboratory of Jiangsu Provincef orAgrobiology, Nanj ing 210014, China) A bstract: A lkaline protease of six representative Fusarium asiaticum isolates w ith different pathogenicities evalua ted by single floret injection (SFI) m ethod were extracted, pu rified and characterized to learn the correlation between en zym e activity and pathogenecity. The results indicated that alkaline protease produced by all selectedF. asaticum isolate w ere identified as serine protease w ith optmi al temperature in the range from 30 to 40 , and optmi al pH value in the range from 7. 5 to 8. 5. The activity of alkaline protease produced byF. asaticum was positively related to the pathogenicity ofF. asaticum. It could be concluded that alkaline protease produced byFusarium asiaticum isolatesw ere positively related to their pathogenicity. K ey words: Fusarium head blight; F usarium asiaticum; pathogen icity;