内皮损伤引起血小板沉积测定方法的建立与应用.PDFVIP

药物评价研究 第 32 卷 第 2 期 2009 年 12 月 - 101 - 内皮损伤引起血小板沉积测定方法的建立与应用 王维亭，徐向伟，何小云，赵专友，汤立达 天津药物研究院 药代与药效动力学重点实验室，天津 300193 摘 要 目的：研究建立血小板沉积模型的方法。方法：采用 99 锝体外血小板标记与光化学血管内膜损伤法建立模型， 99 通过血小板沉积数测定研究模型的应用性。结果：实验结果表明， 锝体外血小板标记率受标记时间影响，最 佳时间为 30 min；洗涤 2 次可将未标记同位素基本分离掉；血小板沉积数表示法较直接 CPM 计数法优越；阳 性对照欣维宁可减少血小板在内膜损伤动脉血管壁上的沉积。结论：通过 99 锝体外血小板标记与光化学血管 内膜损伤建立的血小板沉积方法，科学、可靠，有一定实用性。 关键词 血小板；99 锝；光化学 中图分类号：R965.1 文献标志码：A 文章编号：1674–6376（2009）02–0101–03 Establishment and application of model for platelet deposition induced by endothelail injury WANG Wei-ting, XU Xiang-wei, HE Xiao-yun, ZHAO Zhuan-you, TANG Li-da State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China Abstract Objective: To study the method of the model establishment for platelet deposition. Methods: Using 99mTc labeled platelet and damage of vascular intima with photo- chemistry, the application of model for platelet deposition was studied by the measurement of platelet deposition. Results: The labeling ratio of platelet with 99mTc was affected by the labeling time, the optimal time was 30 min, the unlabeled platelet could be separated from labeled platelet through two times of scrubbing by buffer, the method of the counts of platelet deposition has superiority than that of CPM, the deposition of platelet in damaged vessels with photochemistry could be reduced by Tirofiban in the positive control group. Conclusions: The method of 99mTc labeled platelet in vitro and damage of vascular intima with photochemistry is reliable, and can be used for evaluation of antiplatelet agen