荜澄茄质量的gc指纹图谱分析-中国现代应用药学.pdf

·专 栏· ·中药与天然药· 荜澄茄质量的GC 指纹图谱分析 1 1 2 2* 于双慧 ，姚奇 ，陈晓辉 ，毕开顺 (1.沈阳市化工学校，沈阳 110122 ；2.沈阳药科大学，沈阳 110016) 摘要：目的 建立评价荜澄茄质量的GC 指纹图谱分析方法。方法 采用水蒸气蒸馏法提取挥发油，GC 测定 14 批荜澄 茄药材。气相色谱条件：DB-Wax 石英毛细管色谱柱(30 m ×0.25 mm ，0.25 μm) ，氢火焰离子化检测器(FID) ，进样口温度 250 ℃，检测器温度250 ℃，柱温起始温度为50 ℃，以3 ℃ ·min1 1 1 升温至75 ℃，以10 ℃ ·min 升温至 130 ℃，以5 ℃ ·min 1 升温至240 ℃，维持15 min。载气为氮气，流速为1.0 mL·min ，进样方式为分流进样，分流比10 ∶1，进样量为1 μL 。 结果 依据 14 批药材的指纹图谱数据进行聚类分析，将样品分为2 类，选定其中的 12 批优质样品建立共有模式，相似 度分析结果表明，第Ⅰ类为优质药材，第Ⅱ类为一般品。结论 本法专属性强、重复性好，为科学评价与鉴定荜澄茄药 材质量提供了依据。 关键词：荜澄茄；挥发油；指纹图谱；质量评价 中图分类号：R282.5 ；R917.101 文献标志码：B 文章编号：1007-7693(2011)08-0721-04 Quality Assessment and Identification by GC Fingerprint for Litsae Fructus 1 1 2 2* YU Shuanghui , YAO Qi , CHEN Xiaohui , BI Kaishun (1.Shenyang Chemical Industry School, Shenyang 110122, China; 2.Shenyang Pharmaceutical University, Shenyang 110016, China) ABSTRACT: OBJECTIVE To establish the method of quality assessment and identification of GC fingerprints for Litsae Frctus. METHODS The essential oil from 14 batches of Litsae Frctus was extracted by steam distillation, and determined by GC. The measurement conditions applied were as follows: DB-Wax capillary column (30 m ×0.25 mm, 0.25 μm), FID detector, inlet temperature was 250 ℃, the detector temperature was 250 ℃, and temperature of column was increased linearly from 50 ℃ to 75 ℃ at a speed of 3 ℃ ·min1, from 75 ℃ to 130 ℃ at a speed of 10 ℃ ·min1, from 130 ℃ to 240 ℃ at a speed of 5 ℃ ·min1 (15 min). Carrier gas was nitrogen(1.0 mL·min1). Split ratio was 10 ∶1. Volume was 1 μL. RESULTS The 14 samples were classified by 2 grades according to clu