中药在抗肿瘤药方面的的研究进展.ppt

中药抗肿瘤药的研究进展Review of the effects of TCM on carcinoma cells 郧阳医学院附属人民医院药学部 汪选斌 中药抗肿瘤药的研究进展 中药抗肿瘤的作用机制 中药抗肿瘤的临床应用 思考与展望 中药抗肿瘤的作用机制 诱导细胞分化 诱导细胞凋亡 干扰核酸的合成 抑制微管蛋白的活性 阻滞细胞周期 逆转多药耐药 其它 诱导细胞分化 药物 肿瘤细胞 靶点 淫羊藿苷（Icariin） HL-60 可能与升高cAMP/cGMP比值有关 苦参碱(Matrine) SMMC-7721、 K562、 HL60 诱导分化 当归多糖（Angelica Polysaccharide ） 小鼠移植性肿瘤EC、Hep、S180、Lewis、B16；K562 增值分化 Differentiation of K-562 cells induced by Matrine. A Normal K-562 cells were irregular in shape, mostly round, uneven in size, scarce cytoplasm, and had large and irregular nuclei and several clear nucleoli. B Matrine-induced(0.1mg/m) changes in the morphology of K-562 cells were characterized by decreased cell size, smaller nucleus cytoplasm ratio, condensed chromatin and disappeared nucleoli, and resembled polychromatic normoblasts and orthochromatic normoblasts. Zhang L P,Jiang J K,Tam J W,et a1.Effects of Matrine on Proliferation and Differentiation in K-562 Cells. Leuk Res,2001,25(9):793 诱导细胞凋亡 抑制微管蛋白的活性 药物 肿瘤细胞 靶点 长春新碱(vincristine) BTT739小鼠膀胱癌 使纺锤体微管蛋白变性； 多西紫杉醇(docetaxel) HL－60 促进微管聚合、抑制微管解聚、变性 阻滞细胞周期 药物 肿瘤细胞 靶点 扶正抑瘤汤（黄芪、当归、莪术、墓回头） BTT739小鼠膀胱癌 S期 土贝母苷 HL－60 G2/M 加味四君子汤 HL－60; K562 G0/G1 Martin Filipits. Mechanisms of cancer:mutidrug resistance. Drug Discovery Today: Disease Mechanisms. 2004.1(2):229-234 逆转多药耐药 Drug resistance is a series of process? MDR Defects in apoptotic signalling glutathione Topoisomerase plasma membrane proteins of the ATP-binding cassette (ABC) superfamily 逆转多药耐药 柴胡逆转肝细胞癌多药耐药作用与相关机制的研究 Fig.1　Expression of P-gp in Bel-7402cells (A)Bel-7402 cells.S-P method×400;(B)Bel-7402 cells after BCDC Treatment for 3d. S-Pmethod×400. Fig.2　The level of MDR1,GSTπ and TopoⅡα gene transcriptin Bel-7402 and Bel-7402 cells treated by 390 g/mL BCDC (lane1,2,3,4,5,6 respectively) 盖晓东，曾常茜，洪敏. 柴胡逆转肝细胞癌多药耐药作用与相关机制的研究. 高等学校化学学报 2005.26.(8):　1446-1450 川芎嗪(Ligustrazine，TMP) 川芎嗪联合维拉帕米对人胃癌耐药细胞SGC7901 / ADM多药耐药的逆转作用研究 曲正， 侯培珍， 曲伟， 等.川芎嗪联合维拉帕米对人胃癌耐药细胞SGC7901 / ADM多药耐药的逆转作用研究. 包头医学院学报, 2005 .21(4) HepG2 R-