小鼠胚芽干细胞的分离培养和向肝细胞定向诱导分化的实验分析-人体解剖与组织胚胎学专业论文.docx

第三部分小鼠胚芽干细胞静脉移植在肝内的存活和分化胚芽干细胞静脉在正常肝脏中的存活和分化……中文摘要····························· ······· ··········· ··········……英文摘要···················· ··· ··· ······················· ··· ··· ···……········ ·· ············· ··········· ···· ·· ····· ··· ······ ······ ··……前言材料和方法······················································……·······························································结果……··········································讨论‘· ···· ··········……参考文献，· · ·· · · ······· ···· ········· ··· ··· ·· · ·············· · ·· ··…………·······························································图版小鼠胚芽干细胞静脉移植在损伤肝脏内的存活和分化……中文摘要·························································……英文摘要·························································……·· ······ ······ ·· ···· ··············· ····· ·········· ··· ··········……前言材料和方法································· ······················……································ ······························结果……·······························································……讨论参考文献·························································……··································· ········ ······ ·········· ····图版附录……····························································致谢已发表或待发表的论文····································……学位论文评阅及答辩情况表之山东大学博士学位论文小鼠胚芽干细胞的分离培养和向肝细胞定向诱导分化的实验研究博士研究生刘晓萍导师高英茂专业人体解剖与组织胚胎学中文摘要自从年和分别从胚泡内细胞群和原始生殖细胞成，、功地建立了人类胚胎干细胞系以来干细胞研究已成为当今生命科学领域中的。前沿和热点研究内容主要涉及胚胎干细胞的培养建系诱导干细胞向特定组。，，织细胞的分化及在生物和医学中的应用来源于原始生殖细胞，的胚胎千细胞又称为胚芽干，，、细胞具有与内细胞群来源的胚胎干细胞相同的。生物学特性本文系统地研究了小鼠胚芽干细胞的提取体外扩增胚芽干细。胞向肝细胞的定向诱导分化和胚芽干细胞肝内移植的实验研究、一胚芽干细胞的提取和体外扩增，胚芽干细胞的提取和培养是进行深入研究的前提我们首先进行了培养体。，系的筛选见系列论文的第一部分，，胚芽干细胞与来源于内细胞群的胚胎干细胞一样在体外培养过程中极。，易发生分化为了在体外维持胚胎干细胞的增殖和未分化状态建立适宜的培。养体系是关键胚胎千细胞对体外培养条件要求非常严格不同种属动物的胚。胎干细胞要求的培养条件也不同近年来对来源于内细胞群的胚胎干细胞的培，。养条件和方法进行了广泛的研究积累了丰富的经验但是有关胚芽干细胞的、、一一、、一一。一一一培养方法报道较少本研究对适用于昆明种小鼠胚芽干细胞一的体外培养体系进。、行了筛选观察了基础培养液一、一一、、一一添白血病抑制因子一、一局杯赢成瘫细添、，一一一一、、一件培养液加，尸一一一一丝裂霉素处理的饲养层培养和与少量腹壁组织一成纤维细一胞共培养等、一一一一、山东大学博士学位论文。培养条啊小鼠胚轩渺的影响结胆生恒同种胎鼠‘矍、养对小鼠胚芽干细胞的培养和扩增的效果最好基础培养夜仅添加白血病抑‘一一一