珍珠柴等3种植物提取物对甜菜夜蛾和菜粉蝶的生物活性研究-动物学专业论文.docx

试的6种溶剂提取物中是最显著的，随着药液浓度的降低和作用时间的增加拒食活性呈下降趋势；丙酮提取物在浓度30 g.L—1时的杀卵作用较好，孵化率为16.67%，二氯甲烷提取物杀卵作用较弱。不同浓度6 种溶剂提取物对菜青虫3龄幼虫的触杀活性是有差异性的，其中水提取物的触杀效果较好；甲醇提取物在浓度30g?I/1时48h后对菜青虫幼虫有胃毒活性，校正死亡率仅为51.11%，其余5种提取物胃毒作用均不明显。3.半日花不同浓度的6种溶剂提取物对甜菜夜蛾均有很好的选择性和非选择性产卵忌避作用。其中，乙酸乙酯提取物在浓度200g.LH时，24h的选择性产卵忌避率和非选择性忌避率均高达100%;丙酮提取物和水提取物在浓度200g?L—1处理24h后杀卵效果较显著，孵化率分别为4.44%和5.56%。半日花6种溶剂提取物对甜菜夜蛾3龄幼虫的胃毒活性均不显著。关键词：珍珠柴，牛心朴子，半円花，甜菜夜蛾，菜粉蝶，提取物，生物活性A bstractInthispapersomekindsofextractswereproducedinthewayofcold-soakedprocessandtraditionalwater-decoetumfrom SalsolapasserinaB unge,CynanchumkomaroviiAL.IljinskiandHelianthemum ordosicum endem ictoChina.Differentconcentrationsof6extractsweremadetoexaminetheirbioactivitiesonLaphygmaexiguaandpierisrapaeininhibitingovipositioneffects,theovicidalaction，contacttoxicity，stomachpoisonactivityandantifeedanteffects.The resultsareasfollow s:1.D ifferent concentrationso f 6extractsof S.Passerinashowedevidentantifeedant effects and inhibiting oviposition effects on L .exigua.when theconcentrationofthe acetoneextract,ethanolextractandmethanolextract was200g-L!，theantifeedantrateonL.exiguainchoicetestswas100% in48hours;whentheconcentrationoftheetheracetateextractwas200g*L1，theovipositiondeterrentrate in choice testsw as96.53% in 24 hours.A llkind ofextractshad no evidentactivityoncontacttoxicactionandeggshatchrateofL.exigua,hsdnostomachpoisonactivity.Differentconcentrationsof6extractsofS.Passerinashowedevidentantifeedanteffectsand inhibiting ovipositioneffectsin choicetestson pierisrapae.when theconcentrationoftheacetoneextractwas200g_L1，theovipositiondeterrentratewas90.05% in24hours.All6kindofextractshadevidenteffectsofantifeedantonpierisespecially,theantifeedantrateofmethanolextractandacetoneextractinchoicetests,withaconcentrationof200g*L1,were100% in24hours;Theresult alsoshowedthatallthesampleshadacertainovicidalaction,butthefunctiondecreasedastimeincreasedandconcentrationdecreased;Allkindofextractshadnoevidentactivityoncontacttoxicactionandstomachpoisona