中国明对虾卵黄蛋白原的cdna克隆及表达特征研究-水生生物学专业论文.docx

AbstraCtAbstraCtAsaPreeursorofthem ajor yo lk Pro tein vitellin 砰n)，vitellogenin(Vg) has beenwellstu d ied in vertebratesan d inseets.H ow ev鱿little 15知ow n ab out th eV g gen e in shrim P.Inth e Presen tstu dy，a fu ll一len gth eD N A sequ enee en eodin g V g w as eloned by hom o logouselone an d RA CE m ethod fro m m atUre ovary ofChineses加m P 厂触nne矛闷户ena秘ehl’n ensis，animPortan teornrnercialsPeeiesin aq uaculture.ThiseDNA seq uen ee157956 bP in length ，eon taining a 7761bP oPen re ad in g fram e，whieh eneodes 2587 田刀In o ac id re sidues.Thededueed am ino ac id sequenc e sh ow ed 80-- 95% identity with otherkn own eru staeeanVgs.M ultiPlealignnlentofam ino aeid sequen eesofV gsfro m difl七renterus tac ean ssh ow sth at V gofF ehinensl’sPossessesthehigh estideniitywiththatof尸me馆uiensl’s.DedueedVgContainsaPutativeN一term inalsign alsequenee(l一18)followed byamaturePePtideineluding2569am inoacidresidues.A Potentialeleav agesite(RXRR)that 15reeogn izedbysubtilisin一likeendoProteas es w as ident ified in th e dedueed V g Pree ursor.The ded ueed PeP tide，with aPredietedmoleeularweigh tof281823Da，andaPlof6.13，has 283Positivelycharg edam inoacids (七g+Ly s)an d 311negatively eharged am ino aeids (AsP +Glu).Vg eontainsmorealan ine，glutam ieaeid，valinean d isoleucine(Ala11.56% ，Glu 8.99% ，Va l8.52% an d Ile7.12% )byam ino aeidseom Position an alysis.Vg gen ewas deteetedboth in fem alean d m ales而m P by RT- PCR m etho d.But trans eriPtions haP PenexeeP tionally in o vary an dheP at0Pan creas ofvitellogen sisfem ales.K ey W ords:Fe nne尹pPenaeusehi刀ensis，vitellogenin，eDN A ，eloning，exPression目录翅鱼旦鱼鱼鱼旦国.旦鱼鱼旦旦鱼旦目录第1章绪论… …1.1研究进展… …11.1.1卵黄蛋白和卵黄蛋白原的性质研究进展……11.1.2卵黄蛋白原基因分子克隆的研究进展……昆虫卵黄蛋白原基因克隆的研究进展……，……脊椎动物vg基因研究进展……甲壳动物卵黄蛋白原基因分子克隆的研究进展……71.1.3甲壳动物卵黄蛋白原基因的表达研究进展……91.2研究目的… …121.3研究意义… …12第2章中国明对虾卵黄蛋白原cDN A 的克隆和序列分析…… ，.… … 142.1引言… …142.2材料与方法… …14