纳米细菌在间质性膀胱炎发病机制中作用的初步研究-外科学(泌尿外)专业论文.docx

Methods:IC patients were enrolled in this study according to the criteria of the National Institutes of Health, National Institutes of Diabetes and Digestive and Kidney Diseases (NIDDK). All enrolled patients received a cystoscopy under anesthesia and bladder biopsies were obtained by cold cup technique. The NB were detected with indirectimmunofluorescent staining （IIFS） and and transmission electron microscope （TEM）.Bladder biopsies and urine samples after treatment were cultured for NB, which were identified by indirect immunofluorescent staining, transmission electron microscope and 16s rRNA gene expression.The patients with positive NB were received a combination of intravesical (500 mg mixed with 30 ml sterile normal buffered saline, bladder instillation) and oral tetracycline (500 mg/day, 3 months). OSPI and PUT were used at the beginning and the end of the trial to assess any changes in symptoms.Adult female SD rats were randomly divided into control group, physiological saline group, NB group and anti-NB group respectively. Bladder injection was used to build the model. Targets including urination, ethology observation, correlated inflammation, mast cells and correlated pain were all observed and detected to compare them among all groups. NB were also re-cultured and detected from the bladder tissues.Main results and conclusions:1、Twenty-seven IC patients were successfully enrolled in this study. NB were detected in bladder tissues from IC/PBS patients by IIFS and TEM, which were found tiny, existing in cytoplasm or bladder interstitium and clustered with different sizes. These results demonstrated that NB do exist in the bladder wall of IC/PBS patients.2、13 out of 27 (48.1%) bladder biopsies showed growth of white granular sedimentswhich firmly were identified to be NB. However, the negative controls showed no microbe growth except one analogue of NB. These findings suggest that nanobacterial infection may participate clinical patholog