抑癌基因wwox在胃癌中表达的实验研究-外科学(普外)专业论文.docx

抑癌基因WWOX在胃癌中表达的实验研究 中文摘要抑癌基因WWOX在胃癌中表达的实验研究 中文摘要目的观察WWOX基因mRNA和编码的蛋白在胃癌组织中的表达情况，分析并探讨WWOX基因的表达与胃癌临床病理学指标的关系。方法 采用半定量逆转录一多聚酶链反应(ImPCR)方法检测52例胃癌组织和配 对癌旁正常胃粘膜组织标本中WWOX mRNA表达情况，用免疫组化方法检测相应组 织的蛋白表达情况。结果应用RT-PCR试验方法测定得到WWOX基因的mRNA在胃癌组织中比癌旁正 常胃粘膜组织呈现明显的低表达(Z=5．1409，PO．01)；WWOX mRNA的表达下调 与胃癌分化程度、TNM分期和浸润深度有关(尸n叮)。应用免疫组化染色试验方法得到WWOX蛋白在胃癌组织中比癌旁正常胃粘膜组织明显低表达(Z2值=22．780， 尸口刃)；WWOX蛋白的表达下调与胃癌分化程度、TNM分期、浸润深度和淋巴结转移有关(尸n∞)。WWOX mRNA和WWOX蛋白在胃癌组织中的表达呈正相关性(r=0．606，PO．01)。WWOXmRNA和WWOX蛋白的表达均与胃癌组织分化程度 呈正相关(r值分别为0．427、0．389，尸值均nDJ土与TNM分期和浸润深度呈负相 关(r值分别为-0．287、-0．556，P值均a 05)。 结论WWOX的表达降低可能参与了胃癌的发生、发展，可望作为临床评价胃癌预 后的参考指标及基因治疗的新靶点。关键词 胃癌 抑癌基因 WWOX基因 RT-PCR 免疫组织化学作者：赵荣飞指导老师：曹苇胡浩抑癌基因WWOX在胃癌中表达的实验研究 英文摘要The study on tumor suppressor WWOX expression inhuman stomach carcinomaAbstractObjectiveTo investigate the expression of WWOX mRNA‘and protein in human stomach carcinoma and its relation to clinicopathological and other molecular parameters． MethodsThe expression of WWOX mRNA was determined：by revere transcription-polymerase chain reaction(RT·-PCR)in 52 stomach carcinoma and corresponding non—cancerous gastric mucosa，semi—quantitative of band densities was performed．The WWOX protein expression in 52 stomach carcinoma and corresponding non—Cancerous gastric mucosa was determined by immunohistochemical staining method．Results RT-PCR showed that the expression of WWOX mRNA Was significantly lower in stomach carcinoma than in normal gastric mucosa(Z=5．1409，PO．01)；the expression of WWOX mRNA Was correlated州t11 pathologic differentiation degree，TNM stage and the depth of invasion(尸a∞)．Immunohistochemical staining also showed that theprotein expression of WWOX in stomach carcinoma was significantly lower than innormal gastric mucosa(Z2=22．780，PO．01)；the expression ofprotein Was correlatedwith pathologic differentiation degree，TNM stage，the depth of invasion and lymph node metastasis(PD．∞)．The relationship between WWOX mRNA and protein expression is significant(r=0．606，PO．01)．There are direc