纳米二氧化钛暴露致小鼠生殖毒性及其基因表达的变化-医学生物化学专业论文.docx

Nano-titanium dioxide exposure induced reproductive toxicity and alterations of gene expression in miceAbstractThe dynamical development of the nanotechnology industry has led to the wide-scale production and application of nanomaterials. Among the various nanomaterials, customarily titanium dioxide nanoparticles (TiO2 NPs), owing to their larger surface area to volume ratio and high reactivity, have been used as nontoxic, chemical inert and biocompatible pigment products or photocatalysts in industry, medicine and health, cosmetics, paint industries, and environmental protection. However, the potentially human toxicity and environmental impact of TiO2 NPs have attracted considerable attention with their increased use in industrial applications. In early 2012, the National Research Council of the National Academy of Sciences of USA released environment, health and safety research strategy of engineered nanomaterials (A Research Strategy for Environmental, Health, and Safety Aspects of Engineered Nanomaterials) report. The report presented the need for overall research program to avoid potential risks caused by fast development nanotechnology (/onpinews//onpinews/ newsitem.aspx? RecordID = 13347). Recent studies confirmed that the TiO2 NPs exposure can cause liver, kidney, spleen, lung, and brain damages in animals, and clarify their damage mechanisms, particularly, kidney and brain damages are closely related with function of the reproductive system. Long-term use containing TiO2 NPs cosmetics and long-term living or working in the environment (such as workers in workshop) exposure to TiO2 NPs, the NPs can penetrate through the skin, respiratory and digestive system into the body, and possibly through blood circulation and blood-testis barrier are accumulated in the ovaries and testes, which led to damage of the reproductive system. However, the damage by TiO2 NPs exposure and its mechanism needs to be addressed. Thus, the aims of the present study were to test the