第十章噬菌体资源.ppt

噬菌体资源（ Bacteriophage Resources） Discovery of Bacteriophages The next morning, on opening the incubator, I experienced one of those moments of intense emotion which reward the research worker for all his pains : at the first glance I saw that the culture which the night before had been very turbid, was perfectly clear : all the bacteria had vanished, they had dissolved away like sugar in water. As for the agar spread, it was devoid of all growth and what caused my emotion was that in a flash I had understood: what caused my clear spots was in fact an invisible microbe, a filterable virus, but a virus which is parasitic on bacteria. Felix dHerelle, French-Canadian Bacteriologist. On his discovery of bacteriophages in 1916; Science News 14: 44-59 (1949) Bacteriophage Application of Phage Contamination and Treatment of Phage Bacteriophage Category Virulent phage; Temperate phage or Lysogenic phage; chronically infecting phage Bacteriophagia In liquid medium, Turbid Clear In solid medium，Form plaque Temperate Phage Prophage Lysogenic Bacterium Lysogeny Lysogenic Cycle Application of Phage Identification and Typing of Bacteria Important Tools in Molecular Biology Phage Display Technique Detection of Pathogenic Bacteria Phage Lytic Enzyme to Control Bacteria Infection Phage Therapy in Animals and Agribisiness Bacteriophage Therapy in Humans Indicator Materials Research Vaccine Detection of Pathogenic Bacteria Recombinant phage (1) Luciferase Reporter Phage (LRP) (2) Other Reporter Phage Detection via phage-mediated cell lysis (1) ATP→ Adenylate kinase (2) Elective Conductivity (3)β-Galactosidase Phage Lytic Enzyme to Control Bacteria  Infection Phage Lytic Enzymes Usages 1. Prevent or reduce the colonization of mucosal surfaces will pathogenic bacteria. 2. Control of sepsis and bactermia. 3. Killing biowarfare bacteria Phage Therapy in Animals and Agribisiness prevent