Filtration principles and Filtration GLP 过滤原理及良好的实验室过滤.pdf

Filtration principles and Filtration GLP 过滤原理及 良好的实验室过滤习惯 Pall Filter Corporation 莫凌霄 Mos Mo 2011-4 Hongkong Confidential Confidentiality This presentation is the Confidential work product of Pall Corporation, and some sections or slides may contain Confidential Information of Pall Corporation available to Pall employees only and prepared for the purpose of this meeting. As such, this presentation cannot be copied, published, performed, or redistributed to third parties. Confidential 2 Where have we been to? Shenzhen BGI (Beijing Genomic Institute) 深圳华大基因研究院 Institute of Medical Biology, Chinese Academy of Medical Sciences 中国医学科学研究院医学生物学研究所 Sun Yat-sen University 中山大学 Shenzhen University 深圳大学 South China University of Technology华南理工大学 South China Agricultural University华南农业大学 Shantou University and Medical University汕头大学，汕头医科大学 Guangxi Academy of Science广西科学院 Sun Yat-Sen University first affiliated hospital中山大学第一附属医院 2011 Shenzhen BioTech DownStream Techniques Seminar 2011深圳生物工程下游工艺研讨会 Confidential 3 Outlines提纲 General filtration principles 过滤原理 -Filtration mechanism过滤机理 -Pore size rating孔径定义 How to choose a suitable filtration device for specific application 如何选择一个合适的过滤装置 -filtration problems and solutions 过滤常见问题及解决方法 Ultrafiltration Technique 超滤技术 -a powerful tool to replace dialysis bag替代透析膜的工具 -TFF system for continuous ultrafiltration 连续超滤装置-TFF系统 Confidential 4 BioTech product purification flow chat Culture Media/Addivtives Preparation Venting/Inlet air Vent filtration Down Stream Processing Harvest Recovery (Homogenizing) Conc/Clarification/ Separation/ Purification/Chrom/ Fract