用于基因和抗癌药物控制释放高分子材料研究精选.ppt

靶向性抗癌药物 5-Fu抗癌药物 PM liver-targeting group 肝靶向高分子抗癌药物 * 肝靶向高分子抗癌药物5-Fu- (99mTc –DTPA)-PAEA-PM注射后不同时间在大鼠体内各器官的分布 * 62.5μg/mL Bel-7204体外凋亡率:51% 135μg/mL Bel-7204体外凋亡率:62% Control 肝靶向高分子抗癌药物5-Fu-PAEA-PM对人体肝癌细胞(Bel-7204 )的体外凋亡实验的荧光照片 * P(TMC-co-EEP) Porcine Pancreas Lipase (PPL) Candida Rugosa Lipase (CL) Al(OiPr)3 酶催化开环聚合反应 * Ring-opening copolymerization of TMC and EEP catalyzed by enzymes Mw/Mn Mn Yield (%) Time(d) Molar ratio(TMC:EEP) [E] (mg/mmol) Lipase - 500 - 2 10:5 10 - - 500 - 2 10:1 10 - 1.69 6410 42.1 2 10:5 10 CL 1.83 6440 62.0 2 10:3 10 CL 2.23 7860 75.6 2 10:1 10 CL 2.18 3230 19.8 2 10:5 10 PPL 1.66 8020 60.1 2 10:3 10 PPL 1.71 10250 82.8 2 10:1 10 PPL 2.63 5730 60.2 4 10:2 10 CL 2.97 6010 57.0 3 10:2 10 CL 1.99 6830 50.2 1 10:2 10 CL 2.75 3430 65.2 4 10:2 10 PPL 1.91 3980 72.3 3 10:2 10 PPL 2.47 5970 56.2 1 10:2 10 PPL 1.81 5328 60.2 2 10:2 50 CL 1.87 6500 57.6 2 10:2 25 CL 2.27 6490 58.3 2 10:2 10 CL 2.43 6230 36.5 2 10:2 25 PPL 2.31 9330 68.9 2 10:2 10 PPL * Polymerization catalyzed by PPL immobilized on silica microparticles (PISM)? * Effect of lipase concentration on Mn and yield 0.0 0.5 1.0 1.5 2.0 2.5 5000 10000 15000 20000 25000 30000 35000 40000 45000 50000 Concentration(wt%) Molecular weight 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 Yield(% ) Mn Yield * Time dependence of Mn and yield during polymerization 10 20 30 40 50 60 10000 12000 14000 16000 18000 20000 22000 24000 Time(h) Mn 40 45 50 55 60 65 70 75 80 85 90 95 100 Yield Mn Yield * Dependence of Mn and yield on the immobilized lipase PPL recycling time Recycling Time Lipase Conc. (wt%) Mn Mw/Mn Yield (%) I 1.0 14700 1.92 70 II 1.0 25400 1.77 75 III 1.0 30800 2.11 81 IV 1.0 45200 2.01 78 VI 1.0 61700 1.94 83 VII 1.0 63000 2.53 81 VIII 1.0 64000 2.20 72 VIII 0.1 87400 2.06 69 Reaction temperature: 100℃. Reaction time: 24h * Polymerization of DTC catalyzed by PPL A immobilized on silica microparticles ? B immobilized on silica nanoparticles * Recycling time of IMPPL vs Mn of Poly(DTC