沙眼衣原体感染不孕患者输卵管液细胞因子的测定.doc

沙眼衣原体感染不孕患者输卵管液细胞因子的测定 【摘要】目的探讨沙眼衣原体(CT)感染输卵管性不孕(TFI)患者输卵管液中γ-干扰素（IFN-γ）、肿瘤坏死因子α(TNF-α)和白细胞介素6、8(IL-6、IL-8)的水平及其意义。方法用酶联免疫吸附试验法测定42例CT感染输卵管性不孕(TFI组) 、35 例已生育输卵管正常妇女(对照组) 输卵管液中IFN-γ、TNF-α和IL-6 、IL-8的水平。结果实验组输卵管液中IFN–γ、TNF-α、IL-6、IL-8的含量分别为(228±50)ng/L、(170±48)ng/L、(693±482)ng/L、(781±395)ng/L,均明显高于对照组(190±60)ng/L、(132±35)ng/L、(218±150)ng/L、(224±89)ng/L (P均 0.05) 。结论生殖道CT感染的输卵管性不孕可能与IFN–γ、TNF-α、IL-6、IL-8等多种因素参与的免疫反应有关。 【关键词】 沙眼衣原体 输卵管疾病 不孕 γ-干扰 肿瘤坏死因子α 白细胞介素6 白细胞介素8 Determination of cytokines levels of the tubal fluids in infertility patients due to Chlamydia Trachomatis infection. ZHANG Qun-feng， LUO Zhi-gang， HUANG Yu-Liang. (Second Affiliated Hospital of Nanhua University, Hengyang, 421001, Hunan, P. R. China) Abstract：Objective To determine the levels of interferon（IFN-gamma;）、tumor necrosis factor-alpha (TNF-alpha;)、interleukin-6 (IL-6) and interleukin-8(IL-8) in tubal fluids of tubal factor infertile (TFI) women due to Chlamydia trachomatis (CT) infection and its implication. Methods Forty-two TFI women with CT infection (group TFI) and thirty-five fertile women with normal tubes without CT infection (group control) were enrolled for study. Levels of IFN-gamma;、TNF-alpha;、IL-6 and IL-8 in tubal fluids were measured by enzyme-linked immunosorbent assay. Results The mean values of IFN-gamma;、TNF-alpha;、IL-6、IL-8 were (228＋50)ng/L、(170±48)ng/L、(693±482)ng/L、(781±395)ng/L respectively in group TFI, all higher than those in infertile group control (P0.05). Conclusion Tubal factor infertile (TFI) caused by chlamydia trachomatis (CT) infection connects with the immune reaction which is associated with multi-factor participated. including IFN-gamma;、TNF-alpha;、IL-6、IL-8. Key words：Chlamydia trachomatis; Fallopian tube diseases; Infertility; IFN-gamma;; TNF-alpha;;IL-6; IL-8 沙眼衣原体(CT)感染是近年来主要的性传播疾病之一，与输卵管性不孕密切相关。有研究显示，不孕妇女生殖道沙眼衣原体感染约为24～31%, 输卵管梗阻局部CT感染高达75%～80%［1］。CT引起的输卵管损伤的机制目前主要偏向于免疫病理损伤，并认为与干扰素-gamma;（IFN-gamma;）、肿瘤坏死因子alpha; (TNF-alpha;)和白细胞介素有关。本研究通过检测输卵管液中干扰