毕业设计论文不同处理对巨紫荆种子萌发的影响.doc

南京林业大学 本科毕业设计（论文） 题 目： 不同处理对巨紫荆种子萌发的影响 二O一一 年五月三十日 不同处理对巨紫荆种子萌发的影响 摘要 巨紫荆是一种良好的景观树种，但其发芽率极低。通过不同的方法探索巨紫荆种子休眠和发芽的原因，然后以试验打破种子的休眠，促进其发芽。实验发现，巨紫荆种子最适合于组织培养，且为剥去种皮将胚直接接种在培养基上的发芽率最高，为18.3%。在探索赤霉素与稀土对种子萌发的影响时，发现赤霉素对巨紫荆种子萌发的促进效果最好，仅有的2颗发芽为赤霉素浸泡的种子。内含物测定时，CAT、POD、SOD、可溶性蛋白质的含量、GSH、可溶性糖的含量都是：清水稀土赤霉素；而可溶性淀粉的含量 稀土清水赤霉素的。巨紫荆种皮内含有抑制种子萌发的物质，此物质溶于有机溶质，抑制作用明显。 关键词： 巨紫荆，种子休眠，种子发芽，稀土，赤霉素，切口 Effect of different treatment on germination of Cercis gigantean Cheng et Kengf. seeds Abstracts:Cercis gigantean Cheng et Kengf. is a kind of good landscape tree species, but its germination rate is extremely low. By different methods to explore giant traversing Cercis gigantean seed dormancy and germination of reason, and then based on experimental breaking seed dormancy and promote its germination. Experimental results show that the most suitable for giant traversing cercis chinensis seeds, and tissue culture for strip kind of skin will embryos in the culture medium directly inoculated the highest germination rate for 18.3%. In exploring gibberellic acid and the influence of rare earth to the germination of gibberellic acid, and found that the Cercis gigantean seed germination, best promoting effect of only two single sprout soaking seeds for gibberellic acid. Inclusions, determination of SOD and POD and CAT, soluble protein content, GSH, soluble sugar content are: gibberellic acid; water rare earth But soluble starch content of rare earth gibberellic acid water. Giant traversing cercis chinensis kind of skin contains inhibiting germination substance, this material soluble in organic solute, inhibitory effect is obvious. Keywords:Cercis gigantean., seed germination, seed dormancy, Rare Earth ,Gibberellin treatment, incision 目录 目录 4 1.前言 5 1.1.巨紫荆的介绍 5 1.2.国外对种子发芽和种子休眠的研究 5 1.3.国内对种子发芽和种子休眠的研究 6 1.3.1种子萌发 6 1.3.2种子休眠 7 1.4.稀土和水杨酸对种子的影响 8 1.4.1稀土的作用 8 1.4.2水杨酸的作用 9 1.4.3赤霉素的作用 9 1.5本研究目的及意义 10 2.材料与方法 10 2.1种子来源及种子处理方法 10 2.2种子处理方法 11 2.2.1巨紫荆种植的组织培养 11 2.2.2赤霉素、稀土、清