生物资讯相关应用的研发-计算系统生物与医学试验室.PPT

Our Aims Develop new tools Create aided valued database Apply to systems biology Apply to Biomedicine Reference Identification of Degenerate Motifs Using Position Restricted Selection and Hybrid Ranking Combination, by C. H. Peng etc., to appear in NAR 彭千華 鍾允昇 etc. 臺灣土雞在育種上所面臨的問題 雜交品系 以肉用雞的篩選方式選拔 飼養期長 結果: 平均產蛋數低 飼養成本高 市場競爭力弱 雞群育種 利用血清蛋白質當作篩選標誌 問題: 多少種標誌? 何種標誌? 是否可用濃度的臨界值來作篩選 ? 單一階段或多重階段篩選? 研究蛋白質體當做雞群的篩選 Lezczynski et al.(1985) Relationship of plasma estradiol and progesterone levels in domestic chicken hens. Poult. Sci. 64, 545. Kuo et al.(2005) Proteomic analysis of hypothalamic proteins of high and low egg production strains of chicken. Theriogenology 64, 1490 Huang et al. (2006) Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens. Proteomics 6, 2217 禽類產蛋之生物路徑分析科學農業 (2004), 10月 探討產蛋過程中, 血清蛋白質的角色 血清蛋白質彼此間的關連 串聯哺乳動物及禽類與生殖相關的分子, 製作產蛋相關生物路徑圖 Serum protein marker Ovotransferrin Vitellogenin Apolipoprotein A-I X protein (an IGF-I like protein) Apo VLDL-II Stage selection Exp I : Association of serum protein levels with egg number at two stage. 24 wk (initial egg production) 35 wk (peak egg production) Exp II: Selection strategy at three stage 14 wk (premature stage) 24 wk 35 wk Exp (I)Fig. 1. Egg production rate of TRFCC (n=157). (A) Total egg number of all hens, (B) hens in four groups Fig. 3. Association of relative protein levels with total egg number. Exp II. 篩選策略 臺灣紅羽土雞 A批 (n=77) 2003年七月孵化 B批 (n=78) 2003年九月孵化 Fig. 1. Egg production rate of batch A (n=77) and batch B (n=78) of TRFCC. Code-selection serum protein level serum protein level Code-selectionStep 1: selection 20% of low egg number of birds in batch B of TRFCC Step 2: Transform codes in batch A of birds 結論 雞血清蛋白質濃度的變化除了和產蛋有關外, 也受到環境的影響 將兩批雞的蛋白質濃度轉成分數(score)及序號(Rank),利用密碼轉換方式可以巧妙的找出兩批雞的蛋白質濃度的規則性, 進而可作預測 血清蛋白質在14週和產蛋無關,卻可以在此階段找出低產雞的規則性 利用密碼篩選法可於14wk淘汰19.5%雞隻，其中包含78.