岩柏草总黄酮对大鼠肝损伤保护作用研究.docVIP

岩柏草总黄酮对大鼠肝损伤保护作用研究 　　摘要：目的：探讨卷柏对实验性肝损伤大鼠保护作用机理。方法：采用四氯化碳(CCl4)腹腔注射导致大鼠急性肝损伤的动物模型,用岩柏草总黄酮混悬液灌胃给药,并以水飞蓟宾葡甲胺组为实验对照。结果：岩柏草总黄酮能显著地降低CCl4所致大鼠血清中ALT、AST、NO、PGE2和肝组织中MDA的含量；并可使CCl4所致大鼠肝组织中降低的SOD活性升高，改善肝脏组织的病理损伤。结论：岩柏草对CCl4所致大鼠肝损伤具有保护作用，其作用机理可能与降酶、抗脂质过氧化反应、清除自由基、抑制NO过量成生，降低PGE2含量等有关。 　　关键词：肝损伤；四氯化碳；岩柏草；黄酮 　　中图分类号：R285.5文献标识码：A文章编号：1673-7717(2012)03-0642-02 　　Protective Effect of Total Flavonoids of Selaginella moellendorfii 　　Hieron on Experimental Liver Injury in Mice 　　WANG Fu?gen,ZHUANG Rang?xiao,FANG Hong?ying,ZHANG Yi?qi,XI Jian?jun 　　(The Sixth Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou 310014,Zhejiang,China) 　　Abstract:Objective：To explore the protective effect of selaginella moellendorfii hieron on experimental liver injury in mice.Method:Acute liver injury models were made by carbon tetrachloride (CCl4), and then were intragastric administered with selaginella moellendorfii hieron.Result:The change of the serum contents of ALT, AST, NO, PGE2, hepatic tissue SOD, MDA, and pathological tissue slices of liver were observed.The result showed that total flavonoids of selaginella moellendorfii hieron could significantly decrease the serum ALT, AST, NO, PGE2 of abnormally high levels induced by CCl4, decrease MDA and increase the activity of SOD of liver tissue, and ameliorate the pathological injure of liver. Conclusion:The protective mechanisms of total flavonoids on liver injury may be correlated to reducing the serum levels of ALT and AST, inhibiting lipid peroxide, cleaning free radical, restraining the excess production of NO and lowering PGE2. 　　Key words:liver injury; carbon tetrachloride; selaginella moellendorfii hieron; flavonoids 　　 　　收稿日期：2011-10-09 　　作者简介：王福根（1974-），男，浙江绍兴人，副主任药师，硕士，研究方向：肝病药物的研发。岩柏草，学名江南卷柏，又名石柏、地柏枝、黄疸卷柏，为卷柏科卷柏属植物江南卷柏Selaginella moellendorfii Hieron的干燥全草，具有凉血止血，清热利湿的功效[1]，民间用来治疗肝炎、各种内外出血等疾病。文献报导其化学成分以双黄酮类为主，主要含穗花杉双黄酮[2]。前期的研究显示，通过大孔树脂纯化岩柏草总黄酮为0.89%。其作为我院医院制剂复方茵柏合剂的主药之一，使用多年，疗效确切。为了进一步探讨复方茵柏