柔毛淫羊藿对骨质疏松小鼠作用研究.docVIP

柔毛淫羊藿对骨质疏松小鼠作用研究 　　[摘要] 目的：探讨柔毛淫羊藿对骨质疏松小鼠骨密度、破骨细胞和IL-6的作用。方法：采用切除雌性小鼠双侧卵巢复制骨质疏松整体动物模型，按小鼠体重随机分对照组（蒸馏水 10 ml/kg）、模型组（蒸馏水 10 ml/kg）、DES组（2 mg/kg）、EPM 高剂量组（200 mg/kg）、EPM 中剂量组（100 mg/kg）、EPM低剂量组（50 mg/kg），每组14只。无钙饲料喂养，自然饮水。分别按各组相应药物相应剂量灌胃给药，60 d后测小鼠骨密度、破骨细胞数和IL-6含量。结果：模型组动物呈现明显的骨质疏松表现；给药组动物骨密度增加、破骨细胞数和IL-6表达明显降低，且有统计学意义。结论：柔毛淫羊藿能够增加去卵巢骨质疏松症小鼠的骨密度、降低破骨细胞数和IL-6的表达。 　　[关键词] 柔毛淫羊藿；骨质疏松；IL-6；骨密度 　　[中图分类号] R965 [文献标识码] A [文章编号] 1673-7210（2011）07（a）-055-02 　　 　　Effects of Epimedium pubescens Maxim. on osteoporosis in ovariectomized mouse 　　HAN Xiaodong1, ZHANG Jinjuan2*, REN Guangyou2, ZHANG Guilin2 　　1.Clinical Medicine, Yan′an University Medical College, Shaanxi 716000,China;2.Department of Pharmacology, Guiyang Medical College, Guizhou 550004,China 　　[Abstract] Objective: To explore the effect of EPM on osteoporosis in ovariectomized mouse. Methods: An ovariectomized mouse model was established. These mice were divided into six groups: control group, model group, diethylstilbestrol positive control group, high dose of EPM group, middle dose of EPM group and low dose of EPM group. Each group of 14. 60 days after, bone density, the osteoclast and IL-6 of the bone tissue was measured. Results: The model group animals had the obvious performance of osteoporosis; bone density increased, the osteoclast number and IL-6 were decreasing obviously. Conclusion: EPM can increase the density. EPM has an apparent role of anti-osteoporosis in ovariectomized mice. And its mechanism may be related to the reducing of the osteoclast number and the level of IL-6. 　　[Key words] Epimedium pubescens Maxim.; Osteoporosis; IL-6; BMD 　　 　　骨质疏松症（Osteoporosis，OP）是一种病因病理复杂，以骨量减少，骨的微观结构退化为特征，致使骨的脆性增加易于发生骨折的全身性骨骼疾病。OP的发病率正逐年升高，引起全世界的广??关注。女性绝经后，雌激素缺乏，骨吸收大于骨形成，引起OP，称为绝经后骨质疏松。近年来，众多学者对雌激素及植物雌激素防治OP作用进行了大量研究，取得了重要进展[1]。IL-6是强有力的骨吸收刺激因子[2]。本实验采用去卵巢复制骨质疏松小鼠模型，重点观察柔毛淫羊藿对骨密度、破骨细胞数和IL-6骨表达的影响。 　　1 材料与方法 　　1.1 动物与药材 　　昆明种小鼠，雌性，体重20~22 g，贵阳医学院动物中心。柔毛淫羊藿浸膏，1 g浸膏相当于生药材6.28 g。己烯雌酚（DES）：合肥久联制药有限公司。一抗兔抗