壳聚糖负载硒代胱氨酸纳米粒子制备及其体外抗肿瘤活性研究.docx

暨南大学本科生毕业论文  PAGE \* MERGEFORMAT IV 壳聚糖负载硒代胱氨酸纳米粒子的制备及其体外抗肿瘤活性的研究 摘 要 硒代胱氨酸(SeC)是一种天然的含硒氨基酸，研究证明，SeC作为一种新型高效的抗肿瘤药物在肿瘤化学预防与治疗中发挥着重要的作用。然而，SeC进入肿瘤细胞与其作用需要较长一段时间，细胞渗透性较差。高分子载药纳米粒子是近年来发展起来的新剂型，药物经其包裹后，不仅可以提高药物稳定性，而且还可以大大提高药物的生物利用率。壳聚糖(CS)是一种优良的天然药物载体，具有良好的生物相容性，生物可降解性和无毒性,被广泛地用作医药载体。本文以壳聚糖为载体，戊二醛为交联剂，液体石蜡为乳化体系，制备出粒径小，分散性好，载药率及包封率较高的SeC壳聚糖纳米粒子。最后通过细胞毒性实验，表明以壳聚糖纳米粒子为药物载体能够增强SeC对MCF-7乳腺癌细胞的抑制作用，并增强其体外抗肿瘤活性。 关键词：壳聚糖纳米粒子；乳化交联法；硒代胱氨酸；体外抗肿瘤活性 Preparation and in vitro anticancer activity of Selenocystine-loaded chitosan nanoparticles Abstract Selenocystine (SeC), a naturally occurring selenoamino acid, has been shown to be a novel compound with broad-spectrum anticancer activity. However, it takes a long time for SeC to interact with cancer cells due to its poor solubility and stability. Polymer, a new drug carrier, is widely used in pharmaceutical field. The encapsulation of drug helps to improve drug stability as well as anticancer activity. Chitosan is a popular drug carrier due to its biocompatible, biodegradable and nontoxic properties. In this paper, Chitosan loaded SeC nanoparticles were prepared in emulsion by using glutaraldehyde as crossing agent, span80 as emulsifier and paraffin as oil phase. Lastly, it’s proved that chitosan nanoparticles loaded SeC had greater toxicity and antitumor activity to MCF-7. Key words: Chitosan nanoparticles; cross-linking emulsification; Selenocystine; in vitro anticancer activity  目 录  TOC \o 1-3 \h \z \u  HYPERLINK \l _Toc326067043 摘 要  PAGEREF _Toc326067043 \h I  HYPERLINK \l _Toc326067044 Abstract  PAGEREF _Toc326067044 \h II  HYPERLINK \l _Toc326067045 第一章 绪论  PAGEREF _Toc326067045 \h 3  HYPERLINK \l _Toc326067046 1.1 硒抗癌药物的研究进展  PAGEREF _Toc326067046 \h 3  HYPERLINK \l _Toc326067047 1.2 硒代胱氨酸的研究现状  PAGEREF _Toc326067047 \h 3  HYPERLINK \l _Toc326067048 1.2.1 硒代胱氨酸的性质  PAGEREF _Toc326067048 \h 3  HYPERLINK \l _Toc326067049 1.2.2 硒代胱氨酸的抗癌作用  PAGEREF _Toc326067049 \h 3  HYPER