毕业论文 伤湿胶囊毒理学和药理学实验研究.doc

Experimental studies on Pharmacodynamics and Toxicology of Wetness-evil Capsule ABSTRACT Objective: To assess the security and effectiveness of Wetness-evil capsule through the experimental studies on its pharmacodynamics and toxicology, providing possible theorem and experimental foundation for the capsule’s clinic trial and application. Methods: Pharmacodynamics observed the effects of the drug on swollen of rat metatarsus, swelling of mouse ear, capillary permeability of mouse skin, granuloma in rat, pain in mouse, and cholesterol in mouse adrenal. If 50% lethal dose (LD50) was not measured out when raising mice once with 37.5% concentration of Wetness-evil capsule, assayed maximum tolerance dose (MTD) with the highest concentration 37.5% and the largest volume 0.3 ml/10g by raising mice for 2~3 times. Raised with high, mid-, and low dose of the drug, then rats were measured body weight, blood routine, total protein, blood sugar, liver and renal function, organ index and pathology. Results: Compared to the compared group and Wetness-evil pill, Wetness-evil capsule was proved to be Significant inflammation-diminishing to rat metatarsus, striking inhibition to mouse ear swelling and skin capillary permeability, also distinguished enhance of threshold for pain, twist reduction, and gland cholesterol decrease in adrenal, then no significance in granuloma inhibition. MTD measured to be 232.88g/kg，amounting to 335 times of daily clinical dosage. Chronic toxicant experiment discovered that high and mid dose of the drug resulted in late-proceeded atrophy of thymus gland, no other apparent side effects. Conclusion: Wetness-evil capsule was proved to be little side effect, prefer anti-inflammation and analgesia to acute inflammation, not obvious inhibition to chronic inflammation. The anti-inflammation mechanism was initially confirmed to be concerned with stimulating adrenal cortex. Subject Headings：Rheumatic disease/ted ther Damp