厌氧污泥胞外聚合物（EPS）的提取方法研究.doc

厌氧污泥胞外聚合物（EPS）的提取方法研究 第25卷第12期 2005年12月 环境科学 ActaScientiaeCircumstantiae Vo1.25,No.12 Dec.,2005 罗曦,雷中方,张振亚,等.好氧/厌氧污泥胞外聚合物(EPS)的提取方法研究[J2.环境科学,2005,25(12):1624—1629 LUOXi.LEIZh0ngfang,ZHANGZhenya,eta1.Studyontheextractionofextracellularpolymericsubstances(EPS)fromaerobic/anaerobicsludges[J].Acta ScientiaeCircumstantiae,2005,25(12):1624—1629 好氧/厌氧污泥胞外聚合物(EPS)的提取方法研究 罗曦,雷中方,,张振亚,杉浦则夫 1.复旦大学环境科学与工程系,上海200433 2.筑波大学生命环境科学研究院,日本筑波3058572 收稿日期:2005.05.23修回日期:2005.09.28录用日期:2005-09-29 摘要:采用5种方法对同一污泥在好氧和厌氧条件下的胞外聚合物(EPS)进行了提取试验研究.结果表明,甲醛-NaOH和H2sO法对EPS的提 取产量最高,分别为232.0mg?g-和159.7mg?g～,且无大量细胞自溶发生,是比较有效的提取方法;与对照方法相比较,阳离子交换树脂(CER) 和戊二醛提取法却存在严重缺陷.两种氧环境条件下污泥EPS中蛋白质的含量均最高,占EPS总量的50%～80%;其次为胞外多糖和DNA.污 泥在经由好氧环境到厌氧环境的转变过程中,EPS中主要成分蛋白质和DNA含量降低较为明显,其厌氧/好氧状态下的相应含量比值为O.59— 0.91.试验中也发现,提取步骤和检测手段对结果的影响最大. 关键词:胞外聚合物;污泥;提取方法;好氧;厌氧 文章编号:0253.2468(2005)121624.06中图分类号:X132文献标识码:A Studyontheextractionofextracellularpolymericsubstances(EPS)fromaerobic/ anaerobicsludges LUOXi,LEIZhongfang,ZHANGZhenya2, SUGIURANorio2 1.DepartmentofEnvironmentalScienceandEngineering,FudanUniversity,Shanghai200433 2.GraduateSchoolofLifeEnvironmentalSciences,TsukubaUniversity,Tsukuba3058572,Japan Received23May2005;receivedinrevisedform28September2005;accepted29September2005 Abstract:Effectiveextractionandidentificationproceduresshouldbesetupatthebeginningofextracellularpolymericsubstances(EPS)study.Fiveextraction methodsareutilizedtoinvestigatetheextractionefficienciesforEPSfromthesamesludgeunderaerobicandanaerobicconditions.Theexperimentalresultssuggest thatformaldehyde.NaOHmethodandH2SO4methodareeffectiveinsludgeEPSextraction,inwhichthecontentsofEPSare232.0mg.g～and159.7mg.g～, respectively.Comparedwithcontrolmethod,cationexchangeresin(CER)methodandglutaraldehydemethodhavesomeshortages.Underthesetwodifferent conditions(aerobicandanaerobic),protein,amountingto50to80percentoftotalEPScontent,isthemajorcomponentinsludgeEPS,followedbycarbohydrate andDNA.Duringtheconversionfromaerobicstatetoanaerobicstate,thecontentsofthemai