血脂分析测试的国家指南教程教案.ppt

National Guidelines on Lipid Profile Testing  E-mail:yanshengkai@ Dr. Sheng-kai Yan , PhD Department of Laboratory Medicine, Peking Union Medical College Hospital Professor zhou xin Department of Laboratory Medicine, Zhongnan hospital of wuhan university. Preface Blood lipid analysis has a substantial importance towards the prevention and management of atherosclerosis and coronary heart disease (CHD). It is also being widely applied to the studies of many other related clinical conditions, such as diabetes mellitus, kidney diseases, and metabolic disorders in postmenopausal women. Recently, the Lipid Expert Panel of the Chinese Society of Laboratory Medicine (CSLM) of the Chinese Medical Association (CMA) has come up with the practical guidelines and recommendations on lipid profile testing. lipid profile testing: total cholesterol (TC) triglycerides (TG) high density lipoprotein–cholesterol (HDL-C) low density lipoprotein-cholesterol (LDL-C) apolipoprotein A1 (ApoA1) apolipoprotein B (ApoB) lipoprotein(a) [Lp(a)] The Guidelines include the content as follows, Preface Preanalytical factors affecting lipid test results Methods of lipid analysis Reagent selection criteria and practical guidelines Clinical significance of cutoff values in the interpretation of lipid profile results See: Chin J Lab Med, 2003, 26(3): 182~184 中华检验医学杂志,2003,26(3):182-184 Preanalytical Factors Affecting Lipid Test Results Both serum and plasma are suitable for analysis. Serum is more common and convenient. The result obtained from EDTA plasma should be corrected for the dilution effect by a factor of 1.03. The separated serum should be processed as soon as possible. Samples that could not be analyzed within 24 hours, can be stored at 4℃ for one week or at -20℃ for several months and at -70℃ for at least half a year. Repeated freeze and thaw should be avoided. Suggestions to reduce the preanalytical variations on blood lipid profile testing Methods of Lipid