ⅳ型胶原在贲门癌自体荧光诊断中的意义-significance of type ⅳ collagen in autofluorescence diagnosis of cardiac carcinoma.docx

discriminate cancerous and normal tissues. Through collecting and analyzing thefluorescence signal, the component s of mixture of different fluorophores can be distinguished. So, we must first get deep understanding of fluorophores, which mainly are large biomolecules, and their auto-fluorescence characteristic of biological tissue. Our Researches to compare the distribution of collagen typeⅢ and Ⅳ in mucosa of normal and carcinoma cardiac tissues and to analyze or probe into the influence of changed collagen on auto-fluorescence after canceration and significance of the Collagen type Ⅳ in the Auto-fluorescence diagnosisof cardiac cancer. MethodsUsing immunohistochemical SABC method with monoclonal anti-collagen typeⅢ and Ⅳ, difference in distribution in extra cellular matrix of two types collagen between the normal and carcinoma tissues from 16 cardiac neoplasm patients approved by pathology were measured with semi-quantitative score methods and contrasting with the confocal image at the same time.ResultsResults of immunohistochemical stainingIn normal mucosa, the epithelial basement membrane showed an intense positive reaction for monoclonal anti-collagenⅣ and an intensively stained ring band was formed around the grand basement membrane; collagen typeⅢ formed ring-like structures apparently representative of fibriform collagen immediately adjacent to the epithelial basement membrane. In carcinomatous mucosa, immunoreactions of the carinomatous cell nest was negative, or only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ； The change of collagen typeⅢ was not apparent, or positives reaction in carcinomatous stroma was more intensity than in normal stroma. By Kruskal-Wallis test, there were significant differences in distribution of collagen type Ⅳ between the normal and thecarcinomatous tissues( d0.001＝13.42 , P ＜ 0.001). Whereas, the normal tissue ofcollagentypeⅢcomparingwiththecarcinomatoustissues,therewereno significant (