fti277对nrf2及胃癌细胞增殖 凋亡的影响-effect of fti 277 on proliferation and apoptosis of nrf 2 and gastric cancer cells.docx

摘要目的：研究胃癌细胞中FTI-277对Nrf-2基因扩增及蛋白表达的影响及其对胃癌细胞增殖、凋亡的作用。方法：采用MTT法观察当实验组中加入FTI-277后对胃癌细胞增殖活性的影响；流式细胞仪观察FTI-277对胃癌细胞凋亡的影响。PCR、Westernblot法分别检测实验组与对照组中Nrf-2 基因扩增及蛋白的表达情况。结果：FTI-277 对胃癌细胞增殖活性具有明显的抑制作用，且呈现出剂量依赖性。FTI-277 具有诱导胃癌细胞凋亡的作用，荧光染色可见明显核碎裂、染色体聚集等。加入FTI-277组Nrf-2的mRNA和蛋白的表达量均明显降低，与对照组相比差异有统计学意义（p﹤0.01）。结论：FTI-277 对胃癌细胞的增殖具有抑制作用、并且促进胃癌细胞自身凋亡。Ras 信号转导通路通过调节Nrf-2 基因扩增及蛋白的表达。抑制胃癌细胞增殖，促进胃癌细胞凋亡。关键词：Nrf-2；胃癌；凋亡IIABSTRACTObjective:WeResearchedtheinfluenceofFTI–277totheNrf-2geneamplificationandproteinexpressioninthegastriccancercellsanditseffectongastriccancercellproliferationandapoptosis.Methods:We used MTTmethod to observe the cellsproliferation activity of gastric cancercellswhenaddedFTI-277inexperimentalgroup;Flowcytometrytechniquewasused toobservethegastriccancercellapoptosiswhenaddedFTI-277.PCRandWesternblotmethodwereusedtotesttheNrf-2genesandproteinexpressionintheexperimentalgroupandcontrolgroup.Results:ItwasobviousthatFTI-277hasaninhibitoryeffectontheproliferationactivityofgastriccancercellsandpresentedadosedependent.FTI-277havethefunctionof inducedgastriccancercellapoptosis,andtherewereobviouskaryorrhexis,chromosomefluorescentdyeaggregation,etc.thelevelofmRNAandexpressionof protein of Nrf-2 were significantly reduced when added FTI-277, which wasstatisticallysignificant(p﹤0.01)comparedwiththecontrolgroup.Conclusion:FTI-277couldinhibitedproliferationandpromotedapoptosisofgastriccancercells.Rassignaltransductionpathwayinfluencedtheproliferationandapoptosisof gastriccancercellbyadjustingtheNrf-2geneamplificationandtheproteinexpression.Keywords:Nrf-2;gastriccarcinoma;apoptosisIII目录第1 章引言...............................................................................................................1第2 章材料...............................................................................................................52.1 细胞株.............................................................................................................52.2 主要试剂....................................