galectin7对食管鳞癌细胞株eca109迁移侵袭的影响及其机制-effect of galectin 7 on migration and invasion of esophageal squamous carcinoma cell line eca 109 and its mechanism.docx

英汉缩略语名词对照英文缩写AP英文全称ammonium persulfate中文全称过硫酸铵cDNAComplement deoxyribonucleic acid互补脱氧核糖核酸ddH2ODouble distilled water双蒸水dNTPDeoxyribonucleoside triphosphate脱氧核苷三磷酸EDTAEthylene Diaminetetraacetic acid乙二胺四乙酸EPEppendorf微量离心管Gal-7Galectin-seven半乳糖凝集素-7MMP-9Matrix metalloproteinase-nine基质金属酶-9PMSFPenylmehtanesufonyl fluoride苯甲基磺酰氟PAGEPolyacrylamide gel electrophoresia聚丙烯酰胺凝胶电泳PBSphosphate buffer saline磷酸盐缓冲液PVDFPoiyvinylidene fluoride聚偏二氟乙烯RT-PCRReal-time polymerase实时聚合酶链式反应SDSSoculium dodecyl sulfate十二烷基硫酸钠TEMEDtetramethylethylenediamine四甲基乙二胺TBSTris buffered salineTris盐缓冲液WBWestern Blot蛋白质印迹法1Galectin-7 对食管鳞癌细胞株 Eca-109 迁移侵袭的影响 及其机制摘要目的探讨半乳糖凝集素-7(Galectin-7)是否存在于食管鳞癌 Eca-109 细胞 外及其在食管鳞癌进展中的机制方法(1)利用免疫印迹法检测 Galectin-7 在浓缩细胞细胞上清液中是否 存在。(2)用浓度不同的重组 Galectin-7 处理食管鳞癌 Eca-109 细胞。用 β-乳糖处理食管鳞癌 Eca-109 细胞(3)实时荧光定量 PCR 检测 MMP-9 的基因转录情况，免疫印迹检 测 MMP-9、p38 和 pp38 的蛋白表达情况，细胞划痕观察各组细胞的迁 移情况、MTT 观察各组细胞的增殖情况。结果（1）Eca-109 细胞细胞上清液中存在 Galectin-7。（2）利用重组 Galectin-7 处理细胞后,MMP-9 和 pp38 的表达都有 升高, 而且细胞的迁移力也得到了提高, 但是增殖能力却没有显著的 变化。（3）用 β-乳糖处理后，细胞 MMP-9 的表达下降。2结论Galectin-7 可以被食管鳞癌 Eca-109 细胞分泌至细胞外。Galectin-7 通过激活 p38MAPK 通路增加 MMP-9 的表达,进而影 响 Eca-109 细胞的转移。Galectin-7 的这种作用可被 β-乳糖抑制。关键词: 半乳糖凝集素-7, 食管癌细胞株 Eca-109，基质金属酶-9,p38,pp383THE EFFECTION AND MECHANISM OF GALECTIN-7 CONCERNING METASTASIS AND INVASION OF THE ESOPHAGEAL SQUAMOUS CARCINOMA CELL LINE ECA-109ABSTRACTOBJECTIVETo investigate whether the Galectin-7 exist in the extracellular of esophageal squamous cell carcinoma Eca-109 cells and the mechanism of Galectin-7 in the progress of esophageal squamous cell carcinoma。METHODSDetecting whether the Galectin-7 exist in the extracellular of esophageal squamous cell carcinoma Eca-109 cells by means of Western blot。Treating esophageal squamous cell carcinoma Eca-109 cells with human recombinant Galectin-7 of different concentrations; Treating esophageal squamous cell carcinoma Eca-109 cells with beta lactose。Detecting the expressions of MMP-9 gene transcription by means of Real-time quantitative P