gh促血小板生成机制分析及dtmpgh对辐射损伤小鼠的救治作用研究-mechanism analysis of gh promoting platelet production and research on the therapeutic effect of dt mpgh on radiation-injured mice.docx

5.2材料与方法 845.3结果 885.4讨论 965.5小结 98全文总结 100参考文献 102文献综述 110参考文献 118攻读学位期间的研究成果 125致谢 126缩略语表英文缩写英文全称中文名称TMPthrombopoietin mimetic peptideTPO 模拟肽 rhGHrecombinant human growth hormone重组人生长激素 GHRgrowth hormone receptor生长激素受体hGHhuman growth hormone人生长激素rhTPOrecombinant human thrombopoietin重组人血小板生成素TPOthrombopoietin血小板生成素TPORThrombopoietin recepterTPO 受体dTMPDouble thrombopoietin mimetic peptide二联体 TPO 模拟肽BSAbovine serum albumin牛血清白蛋白CCK-8Cell Counting Kit-8细胞计数试剂盒-8DMSODimethyl Sulfoxide二甲基亚砜FBSFetal bovine serum胎牛血清FITCFluorescein isothiocyanate异硫氰酸酶荧光素G-250coomassie blue G－250考马斯亮蓝 G-250GM-CSFgranulocyte-macrophage colony stimulating factor粒细胞-巨噬细胞集落刺激 因子IL-3Interleukin 3白介素-3IL-11Interleukin 11白介素 11SCFStem cell factor干细胞生长因子MNCMononuclear cells单核细胞ODoptical density光密度值PBSphosphate balanced solution磷酸盐缓冲液PCRpolymerase chain reaction聚合酶链反应PEG-rhMGDFpegylated recombinant human megakaryocyte growth and development factor聚乙二醇化重组人巨核细 胞生长和发育因子coomassie blue R-250考马斯亮蓝 R-250RT-PCRreverse transcription PCR反转录 PCR1SDSsodium dodecyl sulfate十二烷基磺酸钠SDSSDS-polyacrylamide gel electrophoresisSDS-聚丙烯酰胺凝胶电泳PIPropidium isodide碘化丙啶APCAllophycocyanin别藻蓝素PEPhycoerythrin枣红蛋白DAPI4,6-diamidino-2-phenylindole4,6-二脒基-2-苯基吲哚MKsmegakaryocytes巨核细胞LSCMlaser confocal microscopy激光共聚焦FCMFlow cytometer流式细胞仪WBCWhite blood cell白细胞RBCRed blood cell红细胞PLTPlatelet血小板DMdemarcation membrane内膜系统2A study on the mechanism of action of growth hormone on thrombopoiesis and the effect of dTMP-GH fusion protein in treating radiation injury in miceAbstractWith an increasingly wide utilization of ionizing radiation in fields of military, economy, agriculture and medical care, etc, radiation-induced injuries are commonly seen nowadays. Radiation injury of hematopoietic tissue is the critical pathogenic factor for hematopoiesis dysfunction of which the clinical manifestations are dramatic decreases of WBCs, RBCs and platelets in peripheral blood. The dramatic reduction and dysfunction of platelets further caus