glut1 hif1α在肾细胞癌中的表达及临床意义-expression and clinical significance of glut 1 hi f1 α in renal cell carcinoma.docx

协同作用。第二部分第三章 乏氧环境下人肾癌 786-0 细胞株 HIF-1α、GLUT1 的表达及 其临床意义分析目的：研究乏氧环境下，人肾癌 786-0 细胞珠中的 HIF-1α及 GLUT1 在 mRNA 水平及蛋白水平表达的变化及意义，探讨 GLUT1 的表达是否受肿瘤乏氧微环境 的影响。方法：人肾癌 786-0 细胞珠在缺氧条件下培养不同时间（8,16,24h）段后， 分别用 RT-PCR 及 Western blot 的方法检测 HIF-1α和 GLUT1 的 mRNA 转录及 蛋白表达情况。结果：常氧状态下 HIF-1α和 GLUT1 的 mRNA 和蛋白均有一定表达；缺氧 处理一定时间（ 8,16,24h)后， HIF-1 α和 GLUT1 的 蛋 白 表 达 水 平 显 著 升 高 (P0.01) ，同时 786-0 细胞 HIF-1 α mRNA 表达有明显改变 (P0.05) ，而 GLUT1mRNA 表达无差异性(P﹥0.05)。结论：786-0 细胞乏氧后 HIF-1α与 GLUT1 蛋白的表达均上调，说明肿瘤乏 氧微环境 HIF-1α可能上调 GLUT1 的表达。缺氧条件下 786-0 细胞 HIF-1α的表 达在转录和翻译水平均有变化，而 GLUT1 表达变化主要要在翻译水平而非转录 水平。在蛋白水平对 GLUT1 表达进行调控及抑制糖酵解途径可作为肾细胞癌治 疗的新靶点。关键词：肾癌；HIF-1α；GLUT1；低氧培养；786-0 细胞The expression of HIF-1α and GLUT1 in renal cell carcinoma and its clinical implicationsAbstractPartⅠChapterⅠ Preliminary investigation of HIF-1α and GLUT1 expression in renal cell carcinoma（RCC） tissuesChapterⅡThe protein expression and relationships between HIF-1α and GLUT1 in clear cell RCC and its clinical implicationsObjectiveTo analyse preliminary the protein expression of hypoxia inducible factor-1α (HIF-1α) and Glucose transporter-1(GLUT1) in clear cell RCC. Expanding the number of cases, further evaluate its clinical implications. Methods Western blot was applied to detect the differential protein levels of HIF-1α and GLUT1 in tumor tissues and corresponding macroscopically normal kidney tissues within eight patients. All,the,eight,RCC,samples,are,clear,cell,RCC,subtype.Collected,samples,46,of,surgic al,resection,of,renal,cell,carcinoma,specimens,as,the,experimental,group,,and,the,othe r,35,cases,of,para-tumor,,kidney,,tissue,,as,,the,control,,group.,Use,,the,,method,,of,,I mmunohistochemical to detect the expression of HIF-1α and GLUT1, and collected clinical data to study the relationship between their expression levels and characteristics of renal carcinogenesis. Results 1, Western blot displayed a dramatically increased HIF-1α and GLUT1 protein content in cancerous samples than