glypican3 afp cd133联合检测在原发性肝癌诊断中的价值-the value of gly pican 3 afp cd 133 combined detection in the diagnosis of primary liver cancer.docx

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glypican3 afp cd133联合检测在原发性肝癌诊断中的价值-the value of gly pican 3 afp cd 133 combined detection in the diagnosis of primary liver cancer.docx

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glypican3 afp cd133联合检测在原发性肝癌诊断中的价值-the value of gly pican 3 afp cd 133 combined detection in the diagnosis of primary liver cancer

GPC3 protein, the positive rate was 77.2%; AFP, the positive rate was 63.1%; CD133, the positive rate was 42.9%; Serum GCP3 protein and AFP in PHC combined detection rate was 84.2%; serum GPC3 and the CD133 jointed detection rate was 70.2% ; combined of the three, the detection rate was92.9%.The immunohistochemistry results of these tissues showed that GPC3 protein mainly in the cytoplasm and cell membrane was brown. The expression levels of GPC3, AFP and CD133 were significantly higher in PHC than tumor-adjacent tissue and these expression levels in tumor-adjacent tissue(0.158±0.023vs0.137±0.019; 0.118±0.022vs0.114±0.012;0.128±0.023vs0.102±0.013,all P0.05) was higher thannormal liver tissue (0.137±0.019 vs0.057±0.016; 0.114±0.012vs0.085±0.009; 0.102±0.013vs0.087±0.015,均 P0.05),respectively.GPC3 expression was positively correlated with tumor size, pathological grade and cirrhosis, but not with degree oftumor differentiation, tumor number, HBsAg, clinical stage and serum AFP level. CD133 expression was positively correlated with portal vein tunor thrombus and clinical stage, but not with tumor size and serum AFP level. The expression levels of GPC3 and CD133 were higher than that of AFP in PHC (both P0.05). The positive rate of serum GPC3 protein in AFP negative liver cancer was 57.1%, in the AFP positive liver cancer, the positive rate was 88.9%%.Conclusion: The GPC3 protein was highly expressed in serum and tissues of primary liver cancer and it may become a new more effective liver cancer marker for PHC. The positive rate of GPC3 in AFP-negative HCC and it may be an effective diagnostic index of such liver cancer. Combined detection of GPC3, AFP and CD133 is helpful for improving the diagnositic rate of primary liver cancer.Keywords：Glypican-3, Alpha-fetoprotein, CD133, Primary hepatic cancerGlypican-3、AFP、CD133 联合检测在原发性肝癌诊断中的价值华中科技大学同济医学院附属协和医院肝胆外科研究生：杨永飞导师：陈立波（教授）研究背景原发性肝癌（primary hepatic carcinoma, PHC)是世界范围内最常见的恶性肿瘤之一，其恶性程度极高，预后差，进展较快，目前对其治疗手段有限。作为世