转Bt基因棉花种子和棉花籽油的致突变性研究.doc

晡猁 峈潰聆Bt蕨單蹬蹬豽摯蹬豽蚐勤硫?雄昜腔袀曹魂俶ㄛ眕?歎蛌Bt價秪蕨單蹬腔假?俶ㄛ掛妗桄眕遠避鶜健釬峈栠俶袀曹撙ㄛ眕?籵蹬腔蹬豽摯蹬豽蚐釬峈勤桽昜ㄛ肮奀扢秝俶勤桽郪ㄛ眕傖爛猾敕?壎隴笱倯俶苤扷峈妗桄第蹋ㄛ蔚蹬豽煨麼蹬豽蚐莖?侞蹋笢侞庣苤扷ㄛ潰聆苤扷嘎咍岓嗣?綻牉婉ㄗPolychromatic erythrocytes , PCEㄘ峚瞄薹ㄗMicronucleus frequency, MN(ㄘ睿儕赽儈曹薹ㄗSperm malformation rateㄘ﹝妗桄賦彆珆尨ㄛ侞庣蹬豽摯蹬豽蚐侞蹋腔妗桄郪腔峚瞄薹迵秝俶勤桽郪眈掀ㄛ湮嗣杅P0.05ㄛ拸珆翍俶船祑ㄛ衄侐郪P0.05ㄛ筍坳蠅腔峚瞄薹掀秝俶勤桽郪苤ㄛ撈涴侐郪腔峚瞄薹珆翍苤衾秝俶勤桽郪˙迵栠俶勤桽郪眈掀ㄛP0.001ㄛ飲衄憤珆翍船祑﹝奻扴妗桄郪腔儕赽儈曹薹迵秝俶勤桽郪眈掀ㄛP0.05ㄛ拸珆翍俶船祑˙迵栠俶勤桽郪眈掀ㄛP0.05ㄛ衄珆翍俶船祑﹝賦彆桶隴ㄩ婓掛妗桄沭璃狟ㄛBt蕨單蹬迵?籵蹬腔蹬豽摯蹬豽蚐勤苤扷极牉婉睿俶牉婉歙帤珆尨堤袀曹魂俶ㄛ?羶衄撙講虴茼﹝ 壽瑩棵ㄩBt蕨單蹬 蹬豽 蹬豽蚐 岓嗣?綻牉婉峚瞄薹 儕赽儈曹薹 Study on Mutagenicity of Cotton Seed and Cotton Seed Oil of Bt-transgenic Cottons Abstract To evaluate the safety of insect resistant cotton plants,which express a Bacillus thuringiensis protein,the mutagenicity of cotton seed and cotton seed oil on mammals was examined.The raw cotton seed and oil was mixed into mice diet. Using cyclophosphamide as positive control,non-modified cotton seed and cotton seed oil as controls, this study tested the micronucleus frequency in mice bone marrow polychromatic erythrocytes and the sperm malformation rate.The results showed that theres no significant difference between most of the seed groups,oil groups and the negative control(P0.05) or that the micronucleus frequency of the seed and oil groups is significantly lower than the negative control(P0.05).On the other hand,there existed significant difference between the seed groups,oil groups and the positive control (micronucleus test P0.001,sperm malformation test P0.05). To conclude,under the condition of this test,the seed and seed oil of Bt and non-modified cottons showed no cytomutagenicity or genomutagenicity on mice,and dose effect was not observed. Key Words: Bt-transgenic cottons cotton seed cotton seed oil polychromatic erythrocytes micronucleus frequency sperm malformation rate 醴翹 珨﹜?晟.......................................................................................1 媼﹜第蹋睿源楊.....................................................