全自动尿沉渣分析仪在鉴别肾性和非肾性尿液红细胞中作用.docVIP

全自动尿沉渣分析仪在鉴别肾性和非肾性尿液红细胞中作用 　　[摘要] 目的：探讨全自动尿沉渣分析仪对尿中红细胞来源鉴别的临床价值。方法：用AVE-763尿沉渣分析仪分别检测肾小球性（肾性）和非肾小球性（非肾性）红细胞的大小、形态和色度的改变。结果：肾性红细胞大小、形态有明显改变，色度分析结果明显减弱，非肾性红细胞的形态、大小和细胞色度与正常红细胞相似，没有明显改变。正常红细胞放置时间较长后引起细胞皱缩性改变，虽然大小、形态发生了改变，但色度确有所增强。结论：红细胞形态和大小改变不能说明红细胞来源于肾脏，只有同时红细胞色度减弱时才能认为红细胞来源于肾脏。 　　[关键词] 血尿；红细胞形态；全自动尿沉渣分析仪；肾性；非肾性 　　[中图分类号] R446.112[文献标识码]A [文章编号]1673-7210（2010）10（a）-013-04 　　 　　The effects of fully automatic urine cell analyzer in discriminating the renal and non-renal urine RBC 　　LIUFang1, YU Tao1, BAO Liansheng 1, HUANG Yongguo1, WEI Jiong2 　　(1.Department of Laboratory, Wuhan Womens Health Center, Wuhan 430016, China; 2.Joint Office of Wuhan Medical Association，Wuhan 430014, China) 　　[Abstract] Objective: To investigate the clinical value of using the fully automatic urine cell analyzer in discriminating the renal and non-renal urine RBC. Methods: The change of the renal and non-renal urine RBC in size, shape and chroma were detected by AVE-763 fully automatic urine cell analyzer. Results: There were remarkable change of the renal urine RBC in size and shape and obvious decrease in chroma, whereas there was no remarkable change in that of the non-renal RBC. The normal RBC had a crimple change which leaded to the change in size and shape after long time placement, and the chroma of crimple RBC increased. Conclusion: The change of RBC in size and shape couldn′t distinguish the origin (renal or non-renal) of the urine RBC, but going with the decrease in chroma can make sure the renal origin of urine RBC. 　　[Key words] Hematuria; Erythrocyte morphology; Fully automatic urine cell analyzer; Renal; Non-renal 　　 　　血尿是指尿中红细胞排泄异常增多，它是泌尿系统疾病常见的症状，其来源有肾脏和泌尿道。快速、准确鉴别肾小球性血尿与非肾小球性血尿，对于临床诊断及治疗有着重要的作用[1-2]。目前，鉴别肾性与非肾性血尿的方法有普通光学显微镜、相差显微镜、扫描电镜、血液分析仪及尿液有形成分分析仪[3]。笔者应用AVE-763全自动尿沉渣分析仪对尿红细胞形态变化检测进行了分析，探讨红细胞的大小、形态和色度的变化，现报道如下： 　　1 材料与方法 　　1.1仪器 　　 AVE-763全自动尿沉渣分析仪（长沙爱威科技实业有限公司）及配套试剂。 　　1.2材料 　　肾性红细胞来源于我院住院肾科患儿，非肾性红细胞取自我院门诊非肾病患儿。正常红细胞悬液采取身体各项指标均正常的成人女性尿液（月经期）10 ml稀释20倍制成