参附注射液对大鼠心肺复苏后早期全身炎症反应影响.docVIP

参附注射液对大鼠心肺复苏后早期全身炎症反应影响 　　［摘 要］ 目的 探讨参附注射液对大鼠心肺复苏后早期全身炎症反 应的影响。方法 24只SD雄性大鼠随机均分为假手术组、常规对照组、参 附治疗组，采用琥珀酰胆碱窒息合并冰氯化钾致大鼠呼吸心跳骤停―心肺复苏模型，复苏后 6h取标本，检测血清肿瘤坏死因子α(TNF_α)、白介素1β(IL-1β)、血浆可溶性细胞间粘 附分子-1(sICAM_1)和丙二醛(MDA)含量。结果 呼吸心跳骤停大鼠心肺复 苏CPR)后6h血清TNF_α、IL-1β、血浆可溶性ICAM_1和MDA含量较假手术组升高，但参附治 疗组较常规对照组低。结论 大鼠CPR后早期存在全身炎症反应，参附注射 液能减轻CPR后全身性炎症反应。 　　［关键词］ 心肺复苏；全身性炎症反应；参附注射液 　　中图分类号：R541.7；R972 文 献标识码：A 文章编号：1009_816X(2007)04_023 4_02 　　Experimental Study on Protective Effect of Shengfu Injection on Syste mic Inflammatory Response in Rats Early after Cardiopulmonary Resuscitation. HU YU-Jian. The Peoples Hospital of Cixi, Zhejiang 315300, China 　　［Abstract］ Objective To evaluate the effects of shengfu inje ction on systemic inflammatory response in the earlier period after cardiopulmon ary resuscitation (CPR) in rats. Methods 24 adult male SD rats w ere randomly allocated into 3 groups: sham group; routine control group and shen gfu injection treatment group. Cardiac arrest was induced by asphyxia and ice_co ld KCL 0.5mmol/L in rats and resuscitation efforts were begun 5 minutes afterwa rds. The levels of TNF_α and IL_1β in serum and the levels of soluble ICAM_1 ( sICAM_1) and malondialde_hyde (MDA) in plasma were measured at 6 hours after CPR . Results Compared with sham group, all of above biological indi ces increased dramatically at 6h after CPR but those in shengfu injection treatm ent group were lower than in control group. Conclusions The stud y confirmed the systemic inflammatory response in the earlier period after CPR i n rats and shengfu injection could relieve it. 　　［Key words］ Cardiopulmonary resuscitation; Systemic inflammato ry response; Shengfu injection 　　 　　心肺复苏就是缺血再灌注，可产生细胞因子变化，导致全身炎性反应综 合征(SIRS)的发生［1］，是复苏后继发多器官功能不全综合征(PRMODS)的重要原因 。本实验采用大鼠呼吸心跳骤停―心肺复苏模型［2］，通过对复苏后血中TNF_α、I L-1β、ICAM_1等炎性细胞因子检测，观察参附注射液(SF)在复苏后全身炎性反应综合征中 的治疗作用，为复苏后SIRS的治疗提供实验依据。 　　 　　1 材料与方法 　　 　　1.1 实验动物及分组：选择健康Sprague_Dawley雄性大鼠24只，重量256.0～338.0克， 日 龄49～56d，术前禁食12h，由复旦大学医学院实验动物中心提供。随机将其均分为三组，